Anti-Bik 抗体 (ab52182)
Key features and details
- Rabbit polyclonal to Bik
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Bik antibody
Bik 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bik -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Bik. Non-phosphopeptide from around the phosphorylation site of threonine 33 (G-M-T-D-S).
-
ポジティブ・コントロール
- Extracts from A549 cells treated with DMSO, human breast carcinoma tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52182の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 18 kDa.
|
ICC/IF | (1) |
Use a concentration of 1 - 5 µg/ml.
|
特記事項 |
---|
IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 18 kDa. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
-
機能
Accelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX. -
ドメイン
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
細胞内局在
Endomembrane system. Around the nuclear envelope, and in cytoplasmic membranes. - Information by UniProt
-
参照データベース
- Entrez Gene: 638 Human
- Omim: 603392 Human
- SwissProt: Q13323 Human
- Unigene: 475055 Human
-
別名
- Apoptosis inducer NBK antibody
- BBC1 antibody
- Bcl-2-interacting killer antibody
see all
画像
-
All lanes : Anti-Bik antibody (ab52182) at 1/500 dilution
Lane 1 : extracts from A549 cells, treated
with DMSO (0.1%, 10mins)
Lane 2 : extracts from A549 cells, treated
with DMSO (0.1%, 10mins) with blocking peptide
Predicted band size: 18 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted? -
Ab52182 at 1/50 dilution staining Human breast carcinoma tissue, with and without blocking peptide; paraffin embedded.
-
ICC/IF image of ab52182 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52182, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
ab52182 staining Bik in human Jurkat cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X and then blocked using 3% serum for 3 hours at 23°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 647 (pink) used at a 1/1000 dilution.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (9)
ab52182 は 9 報の論文で使用されています。
- Duan N et al. Overexpression of HIPK2 removes the transrepression of proapoptotic genes mediated by the CtBP1-p300-FOXO3a complex and increases the chemosensitivity in osteosarcoma cells. J Cancer 12:1826-1837 (2021). PubMed: 33613771
- Xu T et al. Gene Amplification-Driven Long Noncoding RNA SNHG17 Regulates Cell Proliferation and Migration in Human Non-Small-Cell Lung Cancer. Mol Ther Nucleic Acids 17:405-413 (2019). PubMed: 31310946
- Kabir S et al. The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells. Elife 8:N/A (2019). PubMed: 31294695
- Zhang L et al. KLF4, a miR-32-5p targeted gene, promotes cisplatin-induced apoptosis by upregulating BIK expression in prostate cancer. Cell Commun Signal 16:53 (2018). PubMed: 30176890
- Wang X et al. Synergistic control of sex hormones by 17ß-HSD type 7: a novel target for estrogen-dependent breast cancer. J Mol Cell Biol 7:568-79 (2015). PubMed: 25966904
- Wu J et al. Silencing of Kv1.5 Gene Inhibits Proliferation and Induces Apoptosis of Osteosarcoma Cells. Int J Mol Sci 16:26914-26 (2015). WB ; Human . PubMed: 26569226
- Varadarajan S et al. The transrepression arm of glucocorticoid receptor signaling is protective in mutant huntingtin-mediated neurodegeneration. Cell Death Differ 22:1388-96 (2015). PubMed: 25656655
- Trejo-Vargas A et al. Bik subcellular localization in response to oxidative stress induced by chemotherapy, in Two different breast cancer cell lines and a Non-tumorigenic epithelial cell line. J Appl Toxicol 35:1262-70 (2015). PubMed: 26059411
- Saito Y et al. Identification of therapeutic targets for quiescent, chemotherapy-resistant human leukemia stem cells. Sci Transl Med 2:17ra9 (2010). PubMed: 20371479