Anti-Biglycan 抗体 (ab58562)
Key features and details
- Goat polyclonal to Biglycan
- Suitable for: ICC
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Biglycan antibody
Biglycan 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Biglycan -
由来種
Goat -
アプリケーション
適用あり: ICCmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Sheep, Horse, Dog, Xenopus laevis, Pufferfish, Zebrafish -
免疫原
Synthetic peptide within Human Biglycan aa 350 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P21810 -
ポジティブ・コントロール
- ICC: Human HepG2 and Mouse NIH/3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58562の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC |
Use a concentration of 10 µg/ml.
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特記事項 |
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ICC
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
May be involved in collagen fiber assembly. -
組織特異性
Found in several connective tissues, especially in articular cartilages. -
配列類似性
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
Contains 12 LRR (leucine-rich) repeats. -
翻訳後修飾
The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 403905 Dog
- Entrez Gene: 100033879 Horse
- Entrez Gene: 633 Human
- Entrez Gene: 12111 Mouse
- Entrez Gene: 25181 Rat
- Entrez Gene: 443011 Sheep
- Entrez Gene: 399351 Xenopus laevis
- Omim: 301870 Human
see all -
別名
- BGN antibody
- Biglycan antibody
- Biglycan proteoglycan antibody
see all
画像
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Immunocytochemistry analysis of paraformaldehyde fixed, 0.15% Triton X 100 permeabilized HepG2 cells staining biglycan with ab58562 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2µg/ml (green). Nuclei counterstained with DAPI (blue).
Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml).
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Immunocytochemistry analysis of paraformaldehyde fixed, 0.15% Triton X 100 permeabilized NIH3T3 cells staining biglycan with ab58562 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2µg/ml (green). Nuclei counterstained with DAPI (blue).
Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (2µg/ml).
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (20)
ab58562 は 20 報の論文で使用されています。
- Yin Y et al. Bmi1 regulate tooth and mandible development by inhibiting p16 signal pathway. J Cell Mol Med 25:4195-4203 (2021). PubMed: 33745198
- Zhou C et al. Genomic deletion of TLR2 induces aggravated white matter damage and deteriorated neurobehavioral functions in mouse models of Alzheimer's disease. Aging (Albany NY) 11:7257-7273 (2019). PubMed: 31509519
- Yokoi M et al. Effect of attenuation of fibroblast growth factor receptor 2b signaling on odontoblast differentiation and dentin formation. In Vitro Cell Dev Biol Anim 55:211-219 (2019). PubMed: 30756235
- Murphy S et al. Proteomic profiling of the dystrophin complex and membrane fraction from dystrophic mdx muscle reveals decreases in the cytolinker desmoglein and increases in the extracellular matrix stabilizers biglycan and fibronectin. J Muscle Res Cell Motil 38:251-268 (2017). PubMed: 28803268
- Hsieh CF et al. Periodontal ligament cells as alternative source for cell-based therapy of tendon injuries: in vivo study of full-size Achilles tendon defect in a rat model. Eur Cell Mater 32:228-240 (2016). PubMed: 27763655