製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Biglycan
  • アプリケーション
    適用あり: IHC-Fr, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
    交差が予測される動物種: Sheep, Horse, Dog, Xenopus laevis, Pufferfish, Zebrafish
  • 免疫原

    Synthetic peptide with sequence

    C-DRLAIQFGNYKK

    , from C Terminal amino acids 357-368 of the (human) protein according to NP_001702.1.(Peptide available as ab119271.)

  • ポジティブ・コントロール
    • Rat Skin lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Biglycan peptide (ab119271).
IHC-P Use at an assay dependent dilution.

ターゲット情報

Anti-Biglycan antibody 画像

  • Anti-Biglycan antibody (ab58562) at 0.1 µg/ml + Rat Skin lysate (35µg protein in RIPA buffer).

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab58562 staining Biglycan in mouse skeletal muscle tissue sections by IHC-Fr (frozen sections). Tissue was fixed with acetone and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/250 in PBS with 1% BSA) for 12 hours at 4°C. An Alexa Fluor®594-conjugated donkey anti-goat IgG polyclonal was used as the secondary antibody.

    See Abreview

Anti-Biglycan antibody (ab58562) 使用論文

This product has been referenced in:
  • Zadrozny LM  et al. Study of the development of the mouse thoracic aorta three-dimensional macromolecular structure using two-photon microscopy. J Histochem Cytochem 63:8-21 (2015). Read more (PubMed: 25362141) »
  • Thomas AC  et al. Foam Cell Formation In Vivo Converts Macrophages to a Pro-Fibrotic Phenotype. PLoS One 10:e0128163 (2015). Mouse . Read more (PubMed: 26197235) »

See all 14 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Pulmonary valve leaflet)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Envision Flex Target Retrieval Solution High pH
Permeabilization
No
Specification
Pulmonary valve leaflet
Blocking step
Rabbit Serum (Normal) plus Rabbit F(ab) anti-Sheep IgG (ab136726) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100µg/mL · Temperature: RT°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 07 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (Cartilage)
Specification
Cartilage
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 12 2014

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Aorta)
Specification
Aorta
Treatment
PNGaseF
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 17 2014

Thank you for your email. We do provide the corresponding peptide for ab58562 Anti-Biglycan antibody. The product is ab119271 Biglycan peptide: http://www.abcam.com/ab119271 Unfortunately we do not currently stock the peptide for ab108930. ...

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: low pH 7.0 TRS and high pH 9.0 TRS-9 from Dako 120C for 30sec
Permeabilization
No
Blocking step
DEEB (dual endogenous enzyme block for autostain) as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. Leann Mikesh

Verified customer

投稿 Jul 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skeletal muscle)
Specification
Skeletal muscle
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録