製品の概要

  • 製品名Anti-Biglycan antibody
    Biglycan 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Biglycan
  • アプリケーション適用あり: IHC-Fr, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
    交差が予測される動物種: Sheep, Horse, Dog, Xenopus laevis, Pufferfish (Fugu), Zebrafish
  • 免疫原

    Synthetic peptide with sequence

    C-DRLAIQFGNYKK

    , from C Terminal amino acids 357-368 of the (human) protein according to NP_001702.1.(Peptide available as ab119271.)

  • ポジティブ・コントロール
    • Rat Skin lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab58562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Biglycan peptide (ab119271).
IHC-P Use at an assay dependent dilution.

ターゲット情報

Anti-Biglycan antibody 画像

  • Anti-Biglycan antibody (ab58562) at 0.1 µg/ml + Rat Skin lysate (35µg protein in RIPA buffer).

    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • ab58562 staining Biglycan in mouse skeletal muscle tissue sections by IHC-Fr (frozen sections). Tissue was fixed with acetone and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/250 in PBS with 1% BSA) for 12 hours at 4°C. An Alexa Fluor®594-conjugated donkey anti-goat IgG polyclonal was used as the secondary antibody.

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Anti-Biglycan antibody (ab58562) 使用論文

This product has been referenced in:
  • Zadrozny LM  et al. Study of the development of the mouse thoracic aorta three-dimensional macromolecular structure using two-photon microscopy. J Histochem Cytochem 63:8-21 (2015). Read more (PubMed: 25362141) »
  • Govindarajan P  et al. Bone matrix, cellularity, and structural changes in a rat model with high-turnover osteoporosis induced by combined ovariectomy and a multiple-deficient diet. Am J Pathol 184:765-77 (2014). Read more (PubMed: 24384131) »

See all 13 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Pulmonary valve leaflet)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Envision Flex Target Retrieval Solution High pH
Permeabilization No
Specification Pulmonary valve leaflet
Blocking step Rabbit Serum (Normal) plus Rabbit F(ab) anti-Sheep IgG (ab136726) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100µg/mL · Temperature: RT°C
Fixative Formaldehyde
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投稿 Oct 07 2016

Application Immunohistochemistry (Frozen sections)
Sample Zebrafish Tissue sections (Cartilage)
Specification Cartilage
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative Paraformaldehyde
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投稿 Mar 12 2014

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Aorta)
Specification Aorta
Treatment PNGaseF
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Jan 17 2014

Thank you for your email. We do provide the corresponding peptide for ab58562 Anti-Biglycan antibody. The product is ab119271 Biglycan peptide: http://www.abcam.com/ab119271 Unfortunately we do not currently stock the peptide for ab108930. ...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: low pH 7.0 TRS and high pH 9.0 TRS-9 from Dako 120C for 30sec
Permeabilization No
Blocking step DEEB (dual endogenous enzyme block for autostain) as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 25°C
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Dr. Leann Mikesh

Verified customer

投稿 Jul 06 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skeletal muscle)
Specification Skeletal muscle
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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投稿 Mar 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"