製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to Biglycan
  • アプリケーション
    適用あり: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-369 of Human Biglycan

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab49701 as a replacement.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab54855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 42 kDa.
IHC-P Use a concentration of 1 - 5 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 20801503

ターゲット情報

  • 機能
    May be involved in collagen fiber assembly.
  • 組織特異性
    Found in several connective tissues, especially in articular cartilages.
  • 配列類似性
    Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.
  • 翻訳後修飾
    The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BGN antibody
    • Biglycan antibody
    • Biglycan proteoglycan antibody
    • Bone/cartilage proteoglycan I antibody
    • Dermatan sulphate proteoglycan I antibody
    • DSPG1 antibody
    • PG S1 antibody
    • PG-S1 antibody
    • PGI antibody
    • PGS1_HUMAN antibody
    • SEMDX antibody
    • SLRR1A antibody
    • Small leucine rich protein 1A antibody
    see all

画像



  • Predicted band size : 42 kDa
    Biglycan antibody (ab54855) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
  • ab54855 (2µg/ml) staining biglycan in human spleen using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1/ in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Overlay histogram showing A431 cells stained with ab54855 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54855, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

This product has been referenced in:
  • Mayer C  et al. Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells. Sci Rep 6:37128 (2016). WB ; Human . Read more (PubMed: 27849015) »
  • Martin-Rojas T  et al. iTRAQ proteomic analysis of extracellular matrix remodeling in aortic valve disease. Sci Rep 5:17290 (2015). WB . Read more (PubMed: 26620461) »

See all 10 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - other (Mesenchymal stem cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Mesenchymal stem cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Apr 22 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録