製品の概要

製品の特性

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アプリケーション

Our Abpromise guarantee covers the use of ab1343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 0.3 - 1.0 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 38 kDa).

    A minor band of unknown identity was also consistently
    observed at 50kDa in Jurkat and Placenta.
    Please note that the 50kDa band could represent another isoform of BIF-1 (AAF73017), which also has several consensus glycosylation sites. Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能May be required for normal outer mitochondrial membrane dynamics (PubMed:15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed:11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed:17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed:21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability.
    • 組織特異性Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
    • 配列類似性Belongs to the endophilin family.
      Contains 1 BAR domain.
      Contains 1 SH3 domain.
    • ドメインAn N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
      The SH3 domain is required and sufficient for the interaction with UVRAG.
    • 翻訳後修飾Phosphorylated at Thr-145 by CDK5; this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization.
    • 細胞内局在Cytoplasm. Golgi apparatus membrane. Mitochondrion outer membrane. Cytoplasmic vesicle, autophagosome membrane. Midbody. Association with the Golgi apparatus depends on the cell type (By similarity). Following starvation colocalizes with ATG5 and LC3 autophagy-related protein(s)on autophagosomal membranes (PubMed:17891140).
    • Information by UniProt
    • 参照データベース
    • 別名
      • Bax interacting factor 1 antibody
      • Bax-interacting factor 1 antibody
      • Bif 1 antibody
      • Bif-1 antibody
      • CGI 61 antibody
      • dJ612B15.2 antibody
      • Endophilin B1 antibody
      • Endophilin-B1 antibody
      • PPP1R70 antibody
      • Protein phosphatase 1 regulatory subunit 70 antibody
      • SH3 containing protein SH3GLB1 antibody
      • SH3 domain GRB2 like endophilin B1 antibody
      • SH3 domain-containing GRB2-like protein B1 antibody
      • Sh3glb1 antibody
      • SH3p13 antibody
      • SHLB1_HUMAN antibody
      see all

    Anti-Bif antibody 画像

    • Anti-Bif antibody (ab1343) at 0.3 µg/ml + Jurkat Lysate at 35 µg

      Predicted band size : 38 kDa

    Anti-Bif antibody (ab1343) 使用論文

    This product has been referenced in:
    • Rudolf R  et al. Regulation of nicotinic acetylcholine receptor turnover by MuRF1 connects muscle activity to endo/lysosomal and atrophy pathways. Age (Dordr) 35:1663-74 (2013). WB . Read more (PubMed: 22956146) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab1343.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"