製品の概要

  • 製品名Anti-Bid antibody
    Bid 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Bid
  • 特異性This antibody detects full length (22 kDa) Bid.
  • アプリケーション適用あり: IHC-Fr, IP, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, surrounding amino acid 37 of Human Bid. (Peptide available as ab38270.)

法規制情報

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab2388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 20 - 40 µg/ml.
IP Use a concentration of 5 - 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa.Can be blocked with Bid peptide (ab38270).

ターゲット情報

  • 機能The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
  • 組織特異性Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
  • ドメインIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 翻訳後修飾TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
  • 細胞内局在Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Apoptic death agonist antibody
    • Apoptotic death agonist BID antibody
    • BH3 interacting domain death agonist antibody
    • BH3 interacting domain death agonist p11 antibody
    • BH3 interacting domain death agonist p13 antibody
    • BH3 interacting domain death agonist p15 antibody
    • BH3-interacting domain death agonist p11 antibody
    • BID antibody
    • BID isoform ES(1b) antibody
    • BID isoform L(2) antibody
    • BID isoform Si6 antibody
    • BID_HUMAN antibody
    • Desmocollin type 4 antibody
    • FP497 antibody
    • Human BID coding sequence antibody
    • MGC15319 antibody
    • MGC42355 antibody
    • p11 BID antibody
    • p13 BID antibody
    • p15 BID antibody
    • p22 BID antibody
    see all

Anti-Bid antibody 画像

  • Western blot analysis of Bid in Jurkat cells treated with anti-Fas for 6 hours.

Anti-Bid antibody (ab2388) 使用論文

ab2388 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. Yes, this antibody detects both full length (22 kDa) and cleaved fragments (15 kDa) of Bid. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. I have added an image of a Western blot using this antibody onto the datasheet. For your convenience, I have included a link to the datasheet at the bottom of this email. If you continue to have a problem with this an...

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The immunogen is a synthetic peptide containing 15 amino acids around amino acids 40 of human Bid.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"