The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 93 kDa.
1/10 - 1/50.
May play a role in the dynein-dynactin interactions on the surface of membranous organelles, by associating with these complexes. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.
Belongs to the BicD family.
The fourth coiled coil region is involved in Golgi targeting and in the interaction with DCTN2.
Phosphorylated by NEK9 in vitro.
Golgi apparatus. Cytoplasm > cytoskeleton. In interphase cells mainly localizes to the Golgi complex and colocalizes with dynactin at microtubule plus ends (By similarity). Its localization is dependent on microtubule morphology.
Immunohistochemical staining of BICD2 in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using ab80012, at 1/10 dilution, and a peroxidase-conjugated secondary antibody and DAB staining.