The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
機能May play a role in the dynein-dynactin interactions on the surface of membranous organelles, by associating with these complexes. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.
配列類似性Belongs to the BicD family.
ドメインThe fourth coiled coil region is involved in Golgi targeting and in the interaction with DCTN2.
翻訳後修飾Phosphorylated by NEK9 in vitro.
細胞内局在Golgi apparatus. Cytoplasm > cytoskeleton. In interphase cells mainly localizes to the Golgi complex and colocalizes with dynactin at microtubule plus ends (By similarity). Its localization is dependent on microtubule morphology.