May play a role in the dynein-dynactin interactions on the surface of membranous organelles, by associating with these complexes. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.
Belongs to the BicD family.
The fourth coiled coil region is involved in Golgi targeting and in the interaction with DCTN2.
Phosphorylated by NEK9 in vitro.
Golgi apparatus. Cytoplasm > cytoskeleton. In interphase cells mainly localizes to the Golgi complex and colocalizes with dynactin at microtubule plus ends (By similarity). Its localization is dependent on microtubule morphology.
Detection of Human BICD2 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells using ab117818 at 6 µg/mg of lysate. ab117818 at 0.4 µg/ml was used for subsequent WB detection of the immunoprecipitate.
Detection: Chemiluminescence with exposure time of 30 seconds.