製品の概要

  • 製品名Anti-BHMT antibody [EPR6782]
    BHMT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6782] to BHMT
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to residues near the C terminus of Human BHMT.

  • ポジティブ・コントロール
    • Human fetal liver and fetal kidney lysates; Human kidney and liver tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124992 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

ターゲット情報

  • 機能Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
  • 組織特異性Found exclusively in liver and kidney.
  • パスウェイAmine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
    Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
  • 配列類似性Contains 1 Hcy-binding domain.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Betaine homocysteine methyltransferase antibody
    • Betaine homocysteine S methyltransferase 1 antibody
    • Betaine homocysteine S methyltransferase antibody
    • Betaine--homocysteine S-methyltransferase 1 antibody
    • BHMT antibody
    • BHMT1 antibody
    • BHMT1_HUMAN antibody
    • EC 2.1.1.5 antibody
    • Epididymis secretory sperm binding protein Li 61p antibody
    • HEL S 61p antibody
    see all

Anti-BHMT antibody [EPR6782] 画像

  • All lanes : Anti-BHMT antibody [EPR6782] (ab124992) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 45 kDa
  • ab124992 at 1/250 dilution staining BHMT in paraffin-embedded Human kidney tissues by Immunohistochemistry.
  • ab124992 at 1/250 dilution staining BHMT in paraffin-embedded Human liver tissues by Immunohistochemistry.
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-BHMT antibody [EPR6782] (ab124992) 使用論文

This product has been referenced in:
  • Megger DA  et al. Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study. Mol Cell Proteomics 12:2006-20 (2013). WB ; Human . Read more (PubMed: 23462207) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"