Anti-beta V Tubulin 抗体 - Carboxyterminal end (ab82366)

製品の概要

  • 製品名
    Anti-beta V Tubulin antibody - Carboxyterminal end
    beta V Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to beta V Tubulin - Carboxyterminal end
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human beta V Tubulin (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P07437

  • ポジティブ・コントロール
    • A2058 cytoplasmic lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ELISA 1/1000.

ターゲット情報

  • 機能
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 組織特異性
    Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • 配列類似性
    Belongs to the tubulin family.
  • ドメイン
    The highly acidic C-terminal region may bind cations such as calcium.
  • 翻訳後修飾
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 細胞内局在
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • beta Ib tubulin antibody
    • M40 antibody
    • TBB5_HUMAN antibody
    • TUBB antibody
    • TUBB1 antibody
    • TUBB5 antibody
    • tubulin beta 1 chain antibody
    • Tubulin beta 5 chain antibody
    • Tubulin beta antibody
    • Tubulin beta chain antibody
    • tubulin beta polypeptide antibody
    • Tubulin beta-5 chain antibody
    see all

画像

  • Anti-beta V Tubulin antibody - Carboxyterminal end (ab82366) at 1/50 dilution + A2058 cell line lysates at 35 µg

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

プロトコール

参考文献

ab82366 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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