Anti-beta Tubulin 抗体, prediluted (ab15569)

製品の概要

  • 製品名
    Anti-beta Tubulin antibody, prediluted
    beta Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to beta Tubulin, prediluted
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Guinea pig, Cow, Pig, Saccharomyces cerevisiae, Gerbil, Sea urchin, Fungi
  • 免疫原

    Synthetic peptide:

    NDLVSEYQQYQDATA

    , corresponding to amino acids 416-430 of Human beta Tubulin.

  • ポジティブ・コントロール
    • MAD109 cells, Skin and lung

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15569 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報
    IHC-P: Working dilution, ready to use for 10 minutes at RT.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
    • 組織特異性
      Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
    • 関連疾患
      Cortical dysplasia, complex, with other brain malformations 6
      Skin creases, congenital symmetric circumferential, 1
    • 配列類似性
      Belongs to the tubulin family.
    • ドメイン
      The highly acidic C-terminal region may bind cations such as calcium.
    • 翻訳後修飾
      Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
      Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
      Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
    • 細胞内局在
      Cytoplasm, cytoskeleton.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Beta 4 tubulin antibody
      • Beta 5 tubulin antibody
      • beta Ib tubulin antibody
      • Beta1 tubulin antibody
      • Class I beta tubulin antibody
      • M40 antibody
      • MGC117247 antibody
      • MGC16435 antibody
      • OK/SW cl.56 antibody
      • OK/SWcl.56 antibody
      • TBB5_HUMAN antibody
      • TUBB 1 antibody
      • TUBB 2 antibody
      • TUBB 5 antibody
      • TUBB antibody
      • TUBB1 antibody
      • TUBB2 antibody
      • TUBB5 antibody
      • tubulin beta 1 chain antibody
      • Tubulin beta 2 chain antibody
      • tubulin beta 5 chain antibody
      • Tubulin beta chain antibody
      • Tubulin beta class I antibody
      • tubulin beta polypeptide antibody
      • Tubulin beta-5 chain antibody
      see all

    画像

    • ab15569 staining beta Tubulin in human lung by Immunohistochemistry (FFPE-sections).

    プロトコール

    参考文献

    ab15569 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Valve interstitial cells and valve endothelial cel)
    Gel Running Conditions
    Reduced Non-Denaturing (Native)
    Loading amount
    25 µg
    Specification
    Valve interstitial cells and valve endothelial cel
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: RT°C
    Username

    Hessah Alshammari

    Verified customer

    投稿 Feb 08 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録