製品の概要

  • 製品名Anti-beta Tubulin antibody
    beta Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to beta Tubulin
  • 特異性ab21057 detects a band of ~50kDa in Hela and A431 lysates (data for A431 not shown). These bands are blocked by the addition of the immunizing peptide, ab20775.
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human, African Green Monkey
    交差が予測される動物種: Rat, Chicken
  • 免疫原

    Synthetic peptide derived from within residues 1 - 100 of Human beta Tubulin.

    (Peptide available as ab20775.)

  • ポジティブ・コントロール
    • HeLa whole cell lysate, HeLa nuclear lysate and A431 cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab21057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human beta Tubulin peptide (ab20775).
ICC/IF 1/1000.

ターゲット情報

  • 機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 組織特異性Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
  • 配列類似性Belongs to the tubulin family.
  • ドメインThe highly acidic C-terminal region may bind cations such as calcium.
  • 翻訳後修飾Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 細胞内局在Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta 4 tubulin antibody
    • Beta 5 tubulin antibody
    • BetaTubulin antibody
    • TBB5_HUMAN antibody
    • TUBB antibody
    • TUBB2 antibody
    • TUBB2A antibody
    • TUBB5 antibody
    • tubulin beta 2A antibody
    • Tubulin beta chain antibody
    • Tubulin beta-5 chain antibody
    see all

Anti-beta Tubulin antibody 画像

  •    Ab21057 staining beta-tubulin in asynchronous HeLa cells (green). Cells were counterstained with DAPI (red) to show the nucleus. Secondary antibody: Donkey anti-Goat antibody conjugated to Alexafluor 488 ® (Please refer to the Abreview for further details).

    See Abreview

  • All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa whole cell lysate with Human beta Tubulin peptide (ab20775) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti Goat polyclonal at 1/10000 dilution

    Predicted band size : 50 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)

    ab21057 detects a band of ~ 50kDa in Hela whole cell lysate, HeLa nuclear lysate and A431 lysates (data for HeLa nuclear and A431 not shown). These bands are blocked by the addition of the immunizing peptide, ab20775.

     

     


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa


    Exposure time : 150 seconds

    Western blot image using the Optiblot Reducing Electrophoresis Kit - 10 x 10 cm (4-20%) (ab119220) with the
    Prism Ultra Protein Ladder (ab116028) 5µl used. We recommend using our ECL substrate kit (ab65623) .
    20ug of Lysate per lane and detection using ab21057 diluted to 1ug/ml.
    Lane 1: Hela cell lysate.
    Lane 2: Jurkat cell lysate
    Lane 3: A431 cell lysate
    Lane 4: HEK293 cell lysate
    Lane 5: HepG2 cell lysate.

Anti-beta Tubulin antibody (ab21057) 使用論文

This product has been referenced in:
  • Xu S  et al. ANRIL lncRNA triggers efficient therapeutic efficacy by reprogramming the aberrant INK4-hub in melanoma. Cancer Lett N/A:N/A (2016). WB ; Human . Read more (PubMed: 27461581) »
  • Anand U  et al. Mechanisms underlying clinical efficacy of Angiotensin II type 2 receptor (AT2R) antagonist EMA401 in neuropathic pain: clinical tissue and in vitro studies. Mol Pain 11:38 (2015). ICC/IF . Read more (PubMed: 26111701) »

See all 9 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, adult)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, adult
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

投稿 Aug 08 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (platelet)
Permeabilization Yes - 0.2% Triton X100
Specification platelet
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (platelet)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification platelet
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 21 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (Heart whole cell lysate)
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Loading amount 30 µg
Treatment Doxycyline to induce transgene
Specification Heart whole cell lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Chris Tracy

Verified customer

投稿 Nov 18 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Mouse Tissue lysate - whole (Heart)
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Feb 02 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Human Tissue lysate - whole (Heart)
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jan 30 2015

I have issued a free of charge replacement.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (CV1 (fibroblasts))
Specification CV1 (fibroblasts)
Fixative Methanol
Permeabilization Yes - Methanol
Username

Mr. Nir Drayman

Verified customer

投稿 Aug 16 2012

Laut SwissProt wird dieses Protein ubiquitär expremiert, daher auch im Gehirn. Er wird Ihnen daher wohl nicht nur Neuronen sondern auch die Gliazellen anfärben. Der Antikörper wurde bisher in der ICC aber nicht in der IHC getestet. Wir haben verschi...

Read More

Frau Z befindet sich in ihrem wohlverdienten Urlaub und ist leider erst im Juli wieder zu erreichen.
In der Zwischenzeit werde ich mich gerne um Ihren Fall kümmern.
Vielen Dank für Ihre Antwort und für diese weiteren Informationen.
Es tu...

Read More

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"