製品の概要

  • 製品名Anti-beta Synuclein antibody
    beta Synuclein 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to beta Synuclein
  • 特異性Less than 0.1% cross reactivity against human alpha-synuclein Cross reactivity: This antibody is known to react with human and rat. Not yet tested in other species.
  • アプリケーション適用あり: ICC, IHC-P, IHC-Fr, WBmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide (IEPLMEPEGSYEDPPQE) corresponding to the carboxyl terminal of human beta-synuclein (108- 125) and was conjugated to diphtheria toxoid via a cystine residue added to the carboxyl terminus of the peptide. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
IHC-P 1/500 - 1/2000.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

ターゲット情報

  • 機能Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease.
  • 組織特異性Expressed predominantly in brain; concentrated in presynaptic nerve terminals.
  • 配列類似性Belongs to the synuclein family.
  • 翻訳後修飾Phosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 14 kDa brain-specific protein antibody
    • Beta-synuclein antibody
    • Phosphoneuroprotein 14 antibody
    • PNP14 antibody
    • Sncb antibody
    • Synuclein beta antibody
    • SYUB_HUMAN antibody
    see all

Anti-beta Synuclein antibody 画像

  • ab6165 staining beta synuclein in human occipital cortex.
    Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
    Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-beta Synuclein antibody (ab6165) 使用論文

This product has been referenced in:
  • Helwig M  et al. Brain propagation of transduced a-synuclein involves non-fibrillar protein species and is enhanced in a-synuclein null mice. Brain 139:856-70 (2016). Read more (PubMed: 26719384) »
  • Beyer K  et al. The decrease of {beta}-synuclein in cortical brain areas defines a molecular subgroup of dementia with Lewy bodies. Brain : (2010). WB ; Human . Read more (PubMed: 20959308) »

See all 3 Publications for this product

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