Application
Western blot
Sample
Chlamydomonas reinhardtii Cell lysate - whole cell (n/a)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
n/a
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 30 minute(s) · Temperature: 4°C · Diluent: 1x TBS + 0.1% Tween20 + 2.5% milk
Dilution
1/5000
Detection
Detection method
Novex™ HRP Chromogenic Substrate
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 12, 37, 60 kDa
Positive control
4, 2, and 1 ug/mL CTB standards (~12 kDa)
Additional data
Additional Notes
lane 1: molecular weight marker
lane 2: 4ug/mL CTB
lane 3: 2ug/mL CTB
lane 4: 1ug/mL CTB
lane 5: cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 11 2018