Anti-beta Sarcoglycan 抗体 (ab83699)
Key features and details
- Rabbit polyclonal to beta Sarcoglycan
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-beta Sarcoglycan antibody
beta Sarcoglycan 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to beta Sarcoglycan -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog -
免疫原
Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (
FSTDYETHEFHLPSGVKSLNVQKASTERITSNATSDLNIKVDGRAIVRGN
) of Human beta Sarcoglycan (NP_000223) -
ポジティブ・コントロール
- WB: Human heart and human fetal heart lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab83699の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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機能
Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. -
組織特異性
Highest expression in heart and skeletal muscle. Low expression in brain, kidney, placenta, pancreas and lung. High expression in fetal brain. Also found in fetal lung, kidney and liver. -
関連疾患
Limb-girdle muscular dystrophy 2E -
配列類似性
Belongs to the sarcoglycan beta/delta/gamma/zeta family. -
翻訳後修飾
Disulfide bonds are present. -
細胞内局在
Cell membrane, sarcolemma. Cytoplasm, cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 535372 Cow
- Entrez Gene: 6443 Human
- Entrez Gene: 24051 Mouse
- Entrez Gene: 100009208 Rabbit
- Entrez Gene: 680229 Rat
- Omim: 600900 Human
- SwissProt: A6QP70 Cow
- SwissProt: Q16585 Human
see all -
別名
- 43 kDa dystrophin associated glycoprotein antibody
- 43 kDa dystrophin-associated glycoprotein antibody
- 43DAG antibody
see all
画像
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Anti-beta Sarcoglycan antibody (ab83699) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human heart lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Gel concentration: 12% -
Anti-beta Sarcoglycan antibody (ab83699) at 1 µg/ml + Human fetal heart lysate at 10 µg
Predicted band size: 35 kDa
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab83699 は 1 報の論文で使用されています。
- Scano M et al. CFTR corrector C17 is effective in muscular dystrophy, in vivo proof of concept in LGMDR3. Hum Mol Genet 31:499-509 (2022). PubMed: 34505136