Anti-beta Sarcoglycan 抗体 (ab55683)

製品の概要

  • 製品名
    Anti-beta Sarcoglycan antibody
    beta Sarcoglycan 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to beta Sarcoglycan
  • アプリケーション
    適用あり: WB, IHC-P, ELISA, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 87-197 of Human beta Sarcoglycan

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55683 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 3 µg/ml.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix.
  • 組織特異性
    Highest expression in heart and skeletal muscle. Low expression in brain, kidney, placenta, pancreas and lung. High expression in fetal brain. Also found in fetal lung, kidney and liver.
  • 関連疾患
    Limb-girdle muscular dystrophy 2E
  • 配列類似性
    Belongs to the sarcoglycan beta/delta/gamma/zeta family.
  • 翻訳後修飾
    Disulfide bonds are present.
  • 細胞内局在
    Cell membrane, sarcolemma. Cytoplasm, cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 43 kDa dystrophin associated glycoprotein antibody
    • 43 kDa dystrophin-associated glycoprotein antibody
    • 43DAG antibody
    • A3b antibody
    • Beta SG antibody
    • Beta-sarcoglycan antibody
    • Beta-SG antibody
    • dystrophin-associated glycoprotein, 43-kD antibody
    • LGMD2E antibody
    • Limb girdle muscular dystrophy 2E (non linked families) antibody
    • Sarcoglycan beta antibody
    • sarcoglycan, beta (43kDa dystrophin-associated glycoprotein) antibody
    • SGC antibody
    • SGCB antibody
    • SGCB_HUMAN antibody
    see all

画像

  • beta Sarcoglycan antibody (ab55683) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human heart.
  • beta Sarcoglycan antibody (ab55683) at 1ug/lane + MCF-7 cell lysate at 25ug/lane.
  • Overlay histogram showing A549 cells stained with ab55683 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55683, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

参考文献

ab55683 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your enquiry.

The sarcoglycan protein is located in the cell membrane and expressed in kidney, so the membrane kidney lyaste ab30193 should be suitable for both these sarcoglycan antibodies. However, they may like to consider a ...

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Thank you for your response and for sending some additional information.
After reading through the detailed protocol you forwarded to Abcam, I would like to make the following comments/suggestions. Please would you be so kind to ask your troubled ...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also ...

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Thank you for your enquiry regarding ab104478 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody.
After reading through the detailed protocol you kindly...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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