製品の概要

  • 製品名Anti-Beta Lactamase antibody [8A5.A10]
    Beta Lactamase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8A5.A10] to Beta Lactamase
  • 特異性This antibody specifically recognizes TEM type beta lactamases.
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Escherichia coli
  • 免疫原

    Recombinant full length protein corresponding to Beta Lactamase.
    Sequence:

    MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW

  • 特記事項Dilute in PBS or medium which is identical to that used in the assay system.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 10 µg/ml. Predicted molecular weight: 31.5 kDa. (31.5kDa is the molecular weight of the unprocessed precursor.)

ターゲット情報

  • 関連性The beta lactam antibiotics (penicillins and cephalosporins) are the most frequently used antimicrobial agents. All of the beta lactams are structurally related through the presence of a core beta lactam ring. Bacterial resistance to beta lactams continues to increase, primarily due to the production of beta lactamases. Beta lactamases catalyze the hydrolysis of the beta lactam bond, which destroys antibacterial activity. Bacteria that produce TEM type or SHV type beta lactamases have point mutations in structural genes that have extended the substrate specificity of these beta lactamases. As a result, many of the beta lactamase producing Gram negative pathogens have become multidrug resistant.
  • 別名
    • AmpA antibody
    • AmpC antibody
    • Cephalosporinase antibody

Anti-Beta Lactamase antibody [8A5.A10] (ab12251) 使用論文

This product has been referenced in:
  • Fantappiè L  et al. Antibody-mediated immunity induced by engineered Escherichia coli OMVs carrying heterologous antigens in their lumen. J Extracell Vesicles 3:N/A (2014). WB . Read more (PubMed: 25147647) »
  • Heggeset TM  et al. Combinatorial mutagenesis and selection of improved signal sequences and their application for high-level production of translocated heterologous proteins in Escherichia coli. Appl Environ Microbiol 79:559-68 (2013). WB . Read more (PubMed: 23144128) »

See all 8 Publications for this product

Product Wall

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ab12251 and ab12252 were derived from mice that were immunized with the full-length beta lactamse. The immunogen of ab12251 was recombinant TEM-1 beta-lactamase while ab12252 was native Type IV beta-lactamase from ...

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ab12251's immunogen is 5'-His-tagged E.coli TEM-1 beta lactamase therefore this antibody is specific to TEM-1 beta lactamases. I have compared the sequence similarity between TEM-1 beta lactamase and ab104926 and ...

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Thank you for contacting us on Friday.

As discussed over the phone, ab12251 and ab97678 are both mouse monoclonals against beta-lactamase. Whilst I cannot find any reference of ab97678 being used in detecting beta-galctosidase expressed in Le...

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Thank you for contacting us. The lactamases appear to be well-conserved, and the antibodies ab97678 and ab12251 are likely to react with a variety of them. They may possibly recognize your sequence but the epitopes they recognize have not been mappe...

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To the best of our knowledge, this antibody recognizes all TEM-type beta lactamases. Data is limited to our own work with TEM-1 and reports from customers who have worked with various strains of E. coli. To date, we have not been informed by any of o...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"