Anti-beta III Tubulin (phospho S172) 抗体 (ab76286)


  • 製品名Anti-beta III Tubulin (phospho S172) antibody
    beta III Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to beta III Tubulin (phospho S172)
  • 特異性The ab76286 sequence is identical to similar regions in bI, bII, and bIII tubulin isotypes.
  • アプリケーション適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: a wide range of other species
  • 免疫原

    Synthetic phospho peptide corresponding to amino acid residues around serine 172 of human beta III Tubulin conjugated to KLH

  • ポジティブ・コントロール
    • C2C12 cells; purified brain tubulin treated with ERK2 kinase.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab76286 was cross adsorbed to unphosphorylated beta III Tubulin (Ser 172) peptide before affinity purification using phospho beta III Tubulin (Ser 172) peptide (without carrier).
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab76286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
WB 1/1000. Predicted molecular weight: 38 kDa.
ELISA 1/2000.


  • 機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
  • 組織特異性Expression is primarily restricted to central and peripheral nervous system.
  • 関連疾患Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
  • 配列類似性Belongs to the tubulin family.
  • ドメインThe highly acidic C-terminal region may bind cations such as calcium.
  • 翻訳後修飾Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 細胞内局在Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • beta 3 tubulin antibody
    • beta-4 antibody
    • CDCBM antibody
    • CFEOM3A antibody
    • M(beta)3 antibody
    • M(beta)6 antibody
    • MC1R antibody
    • Neuron specific beta III Tubulin antibody
    • Neuron-specific class III beta-tubulin antibody
    • QccE-11995 antibody
    • QccE-15186 antibody
    • TBB3_HUMAN antibody
    • Tubb 3 antibody
    • TUBB3 antibody
    • TUBB4 antibody
    • Tubulin beta 3 antibody
    • Tubulin beta 3 chain antibody
    • Tubulin beta 4 antibody
    • Tubulin beta III antibody
    • Tubulin beta-3 chain antibody
    • Tubulin beta-4 chain antibody
    • Tubulin beta-III antibody
    see all

Anti-beta III Tubulin (phospho S172) antibody 画像

  • ab76286, at a 1/100 dilution, staining beta III Tubulin in C2C12 cells by Immunoflurescence.
    Image 1: untreated.
    Image 2: in the presence of the phospho peptide.
  • All lanes : Anti-beta III Tubulin (phospho S172) antibody (ab76286) at 1/1000 dilution

    Lane 1 : purified brain tubulin, untreated
    Lane 2 : purified brain tubulin, treated with ERK2 kinase to phosphorylate Ser 172

    Predicted band size : 38 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

Anti-beta III Tubulin (phospho S172) antibody (ab76286) 使用論文

ab76286 has not yet been referenced specifically in any publications.

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