製品の概要

  • 製品名Anti-beta III Tubulin antibody [TU-20] (FITC)
    beta III Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [TU-20] to beta III Tubulin (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性This antibody recognizes the peptide sequence ESESQGPK (amino acids 441-448) of human class III beta tubulin specific for neurones.
  • アプリケーション適用あり: ICC, IHC-FoFrmore details
  • 種交差性
    All (the recognized epitope is conserved within all species).
  • 免疫原

    Synthetic peptide: corresponding to amino acids (C)441-448 (

    ESESQGPK

    ) of beta III Tubulin conjugated to maleimide activated KLH via cysteine added to the N terminus of the neurone specific peptide.

  • 特記事項

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 15mM Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Size exclusion chromatography.
  • ポリ/モノモノクローナル
  • クローン名TU-20
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/40.
IHC-FoFr Use at an assay dependent dilution.

ターゲット情報

  • 機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.
  • 組織特異性Expression is primarily restricted to central and peripheral nervous system.
  • 関連疾患Defects in TUBB3 are the cause of congenital fibrosis of extraocular muscles type 3A (CFEOM3A) [MIM:600638]. A congenital ocular motility disorder marked by restrictive ophthalmoplegia affecting extraocular muscles innervated by the oculomotor and/or trochlear nerves. It is clinically characterized by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. Congenital fibrosis of extraocular muscles type 3 presents as a non-progressive, autosomal dominant disorder with variable expression. Patients may be bilaterally or unilaterally affected, and their oculo-motility defects range from complete ophthalmoplegia (with the eyes fixed in a hypo- and exotropic position), to mild asymptomatic restrictions of ocular movement. Ptosis, refractive error, amblyopia, and compensatory head positions are associated with the more severe forms of the disorder. In some cases the ocular phenotype is accompanied by additional features including developmental delay, corpus callosum agenesis, basal ganglia dysmorphism, facial weakness, polyneuropathy.
  • 配列類似性Belongs to the tubulin family.
  • ドメインThe highly acidic C-terminal region may bind cations such as calcium.
  • 翻訳後修飾Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 細胞内局在Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 別名
    • beta 3 tubulin antibody
    • beta-4 antibody
    • CDCBM antibody
    • CDCBM1 antibody
    • CFEOM3 antibody
    • CFEOM3A antibody
    • FEOM3 antibody
    • M(beta)3 antibody
    • M(beta)6 antibody
    • MC1R antibody
    • Neuron specific beta III Tubulin antibody
    • Neuron-specific class III beta-tubulin antibody
    • QccE-11995 antibody
    • QccE-15186 antibody
    • TBB3_HUMAN antibody
    • Tubb 3 antibody
    • TUBB3 antibody
    • TUBB4 antibody
    • Tubulin beta 3 antibody
    • Tubulin beta 3 chain antibody
    • Tubulin beta 4 antibody
    • Tubulin beta III antibody
    • Tubulin beta-3 chain antibody
    • Tubulin beta-4 chain antibody
    • Tubulin beta-III antibody
    see all

Anti-beta III Tubulin antibody [TU-20] (FITC) 画像

  • Immunohistochemical analysis of innervated frozen Human internal anal sphincter implanted in mice, staining beta III Tubulin with ab25770.

Anti-beta III Tubulin antibody [TU-20] (FITC) (ab25770) 使用論文

This product has been referenced in:
  • Rharass T  et al. Ca2+-mediated mitochondrial reactive oxygen species metabolism augments Wnt/ß-catenin pathway activation to facilitate cell differentiation. J Biol Chem 289:27937-51 (2014). ICC ; Human . Read more (PubMed: 25124032) »
  • Raghavan S  et al. Successful implantation of bioengineered, intrinsically innervated, human internal anal sphincter. Gastroenterology 141:310-9 (2011). IHC-Fr ; Mouse . Read more (PubMed: 21463628) »

See all 3 Publications for this product

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