製品の概要

  • 製品名
    Anti-beta II Tubulin antibody
    beta II Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to beta II Tubulin
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 107-137 of Human beta II Tubulin

  • ポジティブ・コントロール
    • MDA-MB231 cell line lysates, Human brain tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab103667 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 50 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the tubulin family.
  • ドメイン
    The highly acidic C-terminal region may bind cations such as calcium.
  • 翻訳後修飾
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 細胞内局在
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA506K6.1 antibody
    • Beta2 antibody
    • class II beta tubulin isotype antibody
    • class IIa beta-tubulin antibody
    • class IIb beta-tubulin antibody
    • Class IVb beta tubulin antibody
    • dJ40E16.7 antibody
    • DKFZp566F223 antibody
    • FLJ98847 antibody
    • M(beta)2 antibody
    • MGC8685 antibody
    • OTTHUMP00000015956 antibody
    • OTTHUMP00000015964 antibody
    • TBB4B_HUMAN antibody
    • TUBB 2 antibody
    • TUBB 2A antibody
    • TUBB 2C antibody
    • TUBB antibody
    • TUBB PARALOG antibody
    • TUBB2 antibody
    • TUBB2A antibody
    • TUBB2B antibody
    • TUBB2C antibody
    • Tubb4b antibody
    • Tubulin beta 2 antibody
    • Tubulin beta 2 chain antibody
    • Tubulin beta 2A antibody
    • Tubulin beta 2A chain antibody
    • Tubulin beta 2B antibody
    • Tubulin beta 2B chain antibody
    • Tubulin beta 2C antibody
    • Tubulin beta polypeptide antibody
    • Tubulin beta polypeptide 2 antibody
    • Tubulin beta polypeptide paralog antibody
    • Tubulin beta-2 chain antibody
    • Tubulin beta-2C chain antibody
    • Tubulin beta-4B chain antibody
    • tubulin, beta 2A class IIa antibody
    • tubulin, beta 2B class IIb antibody
    • tubulin, beta 4B class IVb antibody
    • Tubulin, beta, class IVB antibody
    • Tubulin, beta-4B antibody
    see all

Anti-beta II Tubulin antibody 画像

  • Anti-beta II Tubulin antibody (ab103667) at 1/100 dilution + MDA-MB231 cell line lysates at 35 µg

    Predicted band size : 50 kDa
  • IHC analysis of beta II Tubulin expression in formalin fixed and paraffin embedded human brain tissue using 1/50 ab103667 followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-beta II Tubulin antibody (ab103667) 使用論文

This product has been referenced in:
  • Rüetschi U  et al. SILAC-Based Quantitative Proteomic Analysis of Diffuse Large B-Cell Lymphoma Patients. Int J Proteomics 2015:841769 (2015). WB . Read more (PubMed: 26060582) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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