製品の概要

  • 製品名Anti-beta I Tubulin antibody
    beta I Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to beta I Tubulin
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Pig
  • 免疫原

    Recombinant protein fragment corresponding to a region within amino acids 174 - 438 of beta I Tubulin.

  • ポジティブ・コントロール
    • WB: 293T, A431, H1299, HeLaS3, HepG2, MOLT4 or Raji cell lysate; ICC/IF: HeLa cells.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab96008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 関連疾患Defects in TUBB1 are a cause of macrothrombocytopenia autosomal dominant TUBB1-related (MAD-TUBB1) [MIM:613112]. It is a congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets.
  • 配列類似性Belongs to the tubulin family.
  • 翻訳後修飾Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 細胞内局在Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2810484G07Rik antibody
    • Beta tubulin 1, class VI antibody
    • Class VI beta tubulin antibody
    • dJ543J19.4 antibody
    • M(beta)1 antibody
    • TBB1_HUMAN antibody
    • TUBB1 antibody
    • Tubulin beta 1 class VI antibody
    • Tubulin beta-1 chain antibody
    • Tubulin, beta 1 antibody
    • tubulin, beta1 antibody
    see all

Anti-beta I Tubulin antibody 画像

  • All lanes : Anti-beta I Tubulin antibody (ab96008) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa
  • Top panel: immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96008 at 1/200 dilution.
    Bottom panel: merged with DNA probe.

Anti-beta I Tubulin antibody (ab96008) 使用論文

This product has been referenced in:
  • Wang H  et al. Multiple mechanisms underlying acquired resistance to taxanes in selected docetaxel-resistant MCF-7 breast cancer cells. BMC Cancer 14:37 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24447372) »

See 1 Publication for this product

Product Wall

Thank you for sending the details.

It is clear that tag is a problem which is interfering with the antibody. It may be that due to stearic hindrance epitope is not fully exposed because of conformational changes in the target protein.

Read More

Thank you for contacting us.

I am sorry we have not tested any of anti tubilin I antibodies in GFP-tubulin 1 tag so we will not be able to recommend any specific antibody. Surely, the heavy tag like GFP affects the binding of antibody to prote...

Read More

I will forward these details on to our Distributor’s Team who would be happy to organise the free of charge replacement. Your customer may be interested to know that if needs be, double staining can be performed with primary antibodies from the sam...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital infor...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"