Anti-beta glucuronidase (GUSB) 抗体 [105] (ab8478)


  • 製品名Anti-beta glucuronidase (GUSB) antibody [105]
    beta glucuronidase (GUSB) 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [105] to beta glucuronidase (GUSB)
  • 特異性This antibody reacts with an non-active center epitope on human placental and liver Beta Glucuronidase. The antibody apparently reacts with the native enzyme only.
  • アプリケーション適用あり: ELISA, IHC-Fr, WB, Othermore details
  • 種交差性
    交差種: Human
    非交差種: Rat



Our Abpromise guarantee covers the use of ab8478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
  • 追加情報AP: 1/10.
    ELISA: 1/10.
    IHC-Fr: 1/10.
    WB: 1/10. The beta-glucuronidase is in its native form a tetramer of MW 280 kDa. Furthermore it can be glycosylated.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Plays an important role in the degradation of dermatan and keratan sulfates.
    • 関連疾患Defects in GUSB are the cause of mucopolysaccharidosis type 7 (MPS7) [MIM:253220]; also known as Sly syndrome. MPS7 is an autosomal recessive lysosomal storage disease characterized by inability to degrade glucuronic acid-containing glycosaminoglycans. The phenotype is highly variable, ranging from severe lethal hydrops fetalis to mild forms with survival into adulthood. Most patients with the intermediate phenotype show hepatomegaly, skeletal anomalies, coarse facies, and variable degrees of mental impairment.
      Note=Mucopolysaccharidosis type 7 is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders.
    • 配列類似性Belongs to the glycosyl hydrolase 2 family.
    • 翻訳後修飾N-linked glycosylated with 3 to 4 oligosaccharide chains.
    • 細胞内局在Lysosome.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Ac2-223 antibody
      • asd antibody
      • Beta G1 antibody
      • Beta glucuronidase antibody
      • Beta-G1 antibody
      • Beta-glucuronidase antibody
      • BG antibody
      • BGLR antibody
      • BGLR_HUMAN antibody
      • FLJ39445 antibody
      • Glucuronidase beta antibody
      • Gur antibody
      • Gus antibody
      • Gus-r antibody
      • Gus-s antibody
      • Gus-t antibody
      • Gus-u antibody
      • GUSB antibody
      • Gut antibody
      • MPS7 antibody
      see all

    Anti-beta glucuronidase (GUSB) antibody [105] (ab8478) 使用論文

    ab8478 has not yet been referenced specifically in any publications.

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    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with mouse has ...

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    Thank you for your email. I'm replying on behalf of Sarah, who is currently out of the office. I have arranged for ab9484 to be sent to you as a free of charge replacement for ab8478. It is being sent to your attention on order# 102313 and you should r...

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    We have just received the following information from the originator of ab8478: The immunogen used to generate the antibody was of human origin. The antibody was not tested against bacterial beta glucuronidase. You can find more information in the fo...

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    The antibody binds to circulating B-glucuronidase.

    Thank you for your patience. I have only just heard back from the antibody's originator. The protein in its native format is a tetramer of approximately 280 kDa. The monomer format is approximately 74 kD. This can deviate a little bit on the gel depend...

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    The MW of this antibody is 150 kD. This antibody reacts in WB with a single protein of approximately 280 kDa.

    Cross reactivity with E.coli beta glucuronidase has not been tested, but as the antibody does not cross react with the rat enzyme I am doubtful that it will work with a bacterial enzyme.

    The antibody will not cross-react with rat, so we do not think that it is likely to cross react with mouse.

    It ahs been tested at 1 in 10 but as you say, this is high and probably wasteful. We would therfore recommend that you titrate the antibody at 1 in 10, 1 in 50, 1 in 100 and 1 in 500 in your particular application.