beta Galactosidase protein (ab7981)

製品の概要

製品の詳細

  • 由来Natural
  • 由来Escherichia coli

特性

Our Abpromise guarantee covers the use of ab7981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 製品の状態Lyophilised
  • 備考Unit Definition: One Unit hydrolyzes one micromole fo p-nitrophenyl-B-D-galacotpyranoside per minute at 25deg.C, pH 7.5 Minimum Activity: >=50 units per mg dry weight Molecular weight: 540,000 Optimum pH: 6-8. Extinction coefficient: E(1%, 280)= 20.9
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C.

    n/a

  • 再構成Reconstitute in 0.1 M Sodium/potassium phosphate, pH 7.0.

関連情報

  • 別名
    • Beta D galactosidase
    • Beta gal
    • Beta galactosidase
    • ECK0341
    • JW0335
    • Lactase
    • lacZ
    see all
  • 関連性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • 細胞内局在Cytoplasmic

プロトコール

beta Galactosidase protein (ab7981) 使用論文

ab7981 has not yet been referenced specifically in any publications.

Product Wall



ab7981:

It is an active protein, the assay we use to determine activity is Beta galactosidase assay. We have not sequenced this product but it is sourced from E coli and should be similar to P00722 (http://www.uniprot.org/uniprot/P0...

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Thank you for your enquiry and interest in our products.

I can confirm that these two products (ab7981 and ab79449) are not His-tagged. I have checked our catalogue and currently we only sell non-tagged beta Galactosidase protein.

I...

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Thank you for contacting Abcam earlier today.

For lot * there are 165 u/mgDW (dry weight) in this lot.

If there is anything else I can help you with, please let me know.

Thank you for contacting us yesterday.

I am sorry to hear you are experiencing difficulties with one of our products. As discussed over the phone, I'd like to try and help you to improve the results currently obtained. In order to do this, c...

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Thank you for your call today and for your question about ab7981.

The buffer recommended for reconstitution is based on the assay described in the following reference:

Craven et al. (JBC, 240, 2468,1965)

The buffer is 0.1 ...

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Thank you for contacting us. The current lot we have in stock, GR20915-2, has 51.0 units activity per milligram dry weight, so you should receive 98 mg. Other lots will vary in the unit activity per milligram. We suggest reconstituting at a min...

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Thank you for your inquiry. I am afraid that these products, ab7981 and ab79449, does not contain a His tag. If there is anything else that I can help you with, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"