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Our Abpromise guarantee covers the use of ab116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. PubMed: 23272238|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 20431063|
|WB||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
ab116 staining beta Galactosidase in Human primary HGG aggregate sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citrate buffer for 40 minutes. Samples were incubated with primary antibody (1/1000) for 1 hour at room temperature. A HRP-conjugated Rabbit anti-mouse IgG was used as the secondary antibody.