Anti-beta Galactosidase 抗体 [BGAL01] (ab3291)

製品の概要

  • 製品名Anti-beta Galactosidase antibody [BGAL01]
    beta Galactosidase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [BGAL01] to beta Galactosidase
  • アプリケーション適用あり: IP, WBmore details
  • 免疫原

    Full length protein (E. coli).

  • ポジティブ・コントロール
    • Purified recombinant / beta Galactosidase protein or transfected cell lines expressing beta Galactosidase tagged proteins.
  • 特記事項Molecular weight of beta Galactosidase = ~ 116 kDa.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.40
    Constituents: 0.0268% PBS, 0.2% BSA
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名BGAL01
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
WB
  • 追加情報Immunoprecipitation (with protein G): use at a concentration of 2µg / mg protein lysate.
    Western Blotting: use at a concentration of 1µg / ml for 2hrs at RT.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    This antibody may be used for detection of beta Galactosidase expressed by the E.coli lacZ geneencoded in many cloned gene sequences, and servesas an indicator for fusion proteins encoded by aninserted DNA sequence.
  • ターゲット情報

    • 関連性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
    • 細胞内局在Cytoplasmic
    • 別名
      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • ECK0341 antibody
      • JW0335 antibody
      • Lactase antibody
      • lacZ antibody
      see all

    Anti-beta Galactosidase antibody [BGAL01] (ab3291) 使用論文

    ab3291 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab3291.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"