Anti-beta Galactosidase 抗体 [BG-02] (ab1047)


  • 製品名Anti-beta Galactosidase antibody [BG-02]
    beta Galactosidase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [BG-02] to beta Galactosidase
  • アプリケーション適用あり: WB, ICC, ICC/IFmore details
  • 免疫原

    Full length native protein (purified) (E. coli).

  • エピトープRecognizes a non-catalytic centre epitope of Beta-galactosidase from E. coli.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度>95% by SDS-PAGE
  • 特記事項(精製)Purified from ascites by precipitation methods.
  • ポリ/モノモノクローナル
  • クローン名BG-02
  • アイソタイプIgG1
  • 研究分野


Our Abpromise guarantee covers the use of ab1047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. Used at a dilution of 1/50 for 18 hrs on mouse mammary cells (see Abreview for further information).


  • 関連性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • 細胞内局在Cytoplasmic
  • 別名
    • Beta D galactosidase antibody
    • Beta gal antibody
    • Beta galactosidase antibody
    • ECK0341 antibody
    • JW0335 antibody
    • Lactase antibody
    • lacZ antibody
    see all

Anti-beta Galactosidase antibody [BG-02] 画像

  • Anti-beta Galactosidase antibody [BG-02] (ab1047) at 1 µg/ml + Purified E. coli beta-galactosidase

    Goat anti-mouse IgG (H+L)-HRP conjugate

    Performed under non-reducing conditions.

Anti-beta Galactosidase antibody [BG-02] (ab1047) 使用論文

This product has been referenced in:

See all 7 Publications for this product

Product Wall

Application IHC - Wholemount
Sample Mouse Tissue (Retina)
Specification Retina

Abcam user community

Verified customer

投稿 Aug 13 2015

We also do not have electronic access to the publication

Folia Biol (Praha). 1992;38(6):350-7.

A novel panel of monoclonal antibodies against beta-galactosidase of Escherichia coli and its versatility for detection of reco...

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The Mouse monoclonal [BG-02] to beta Galactosidase, ab1047, should be able to detect the beta Galactosidase with the same sequence as that expressed in E. coli. This should work whether the protein is overexpressed or not. It should also work with beta...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ca...

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If Chicken secondary is a problem then I would suggest choosing other beta galactosidase antibodies from the catalogue, with other host e.g. rabbit as ab4761. I hope this will help.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (whole brain)
Loading amount 50 µg
Specification whole brain
Treatment Samples from wild-type (control) mice and gene-trap mice expressing b-geo fusion proteins
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Dr. Teresa Gunn

Verified customer

投稿 Jul 31 2007

Thank you for your enquiry. This antibody shows no cross-reactivity with other E. coli proteins and can be used for identification and characterization of beta-galactosidase fusion proteins expressed in lambda expression vectors. It was successfull...

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Thank you for your enquiry. I am sorry I can not answer the question; however, I did find the following information in a product specific publication for this clone by Slavickova et al., PubMed ID 1284241. In this publication I have found these ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mammary)
Specification Mammary
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

投稿 Apr 05 2006

Thank you for your enquiry. To our knowledge, our beta Galactosidase antibody [BG-02]has yet to be tested by immunohistochemistry or on guinea pig tissue. All tested applications are specified on Abcam product datasheets. Should you require fur...

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