製品の概要

  • 製品名Anti-beta Galactosidase antibody
    beta Galactosidase 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to beta Galactosidase
  • 特異性Detects recombinant beta galactosidase enzyme by Western blotting.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Escherichia coli
  • 免疫原

    Recombinant full length protein of Escherichia coli beta Galactosidase.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.025% Sodium azide
    Constituent: PBS

    This product may contain up to 3% BSA depending on the batch. For specific batch formulations please contact us.
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab12081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 116 kDa.

ターゲット情報

  • 関連性Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.
  • 細胞内局在Cytoplasmic
  • 参照データベース
    • 別名
      • Beta D galactosidase antibody
      • Beta gal antibody
      • Beta galactosidase antibody
      • ECK0341 antibody
      • JW0335 antibody
      • Lactase antibody
      • lacZ antibody
      see all

    Anti-beta Galactosidase antibody 画像

    • Anti-beta Galactosidase antibody (ab12081) at 1.5 µg/ml + Beta Galactosidase Recombinant at 0.1 µg

      Secondary
      Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 116 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)


      Exposure time : 90 seconds

    Anti-beta Galactosidase antibody (ab12081) 使用論文

    This product has been referenced in:

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Escherichia coli Cell lysate - whole cell (bact beta-gal protein expressed in rat epithelial)
    Loading amount 20 µg
    Specification bact beta-gal protein expressed in rat epithelial
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Dr. Silvia Finnemann

    Verified customer

    投稿 Aug 13 2012

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not with the full amount as stated on the datasheet and for the inconvenience...

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    Thank you for your phone call.

    As we discussed, I recommend using aqueous mounting media for fluorescent detection, such as ab104139. These media are often specially formulated to preserve the fluorescence and simultaneously counterstain with...

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    Thank you for your email. Unfortunately we only have the same lot in stock, would you like a replacement from the same lot or an alternative product? We will be able to organise the replacement with Cedarlane. Looking forward to your re...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"