Anti-beta Dystroglycan 抗体 [EP2200Y] (ab62373)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2200Y] to beta Dystroglycan
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-beta Dystroglycan antibody [EP2200Y]
beta Dystroglycan 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2200Y] to beta Dystroglycan -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Mouse brain, heart, and skeletal muscle tissue lysates; HepG2, HEK293T, Neuro2a and NIH/3T3 whole cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2200Y -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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KO cell lines
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KO cell lysates
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62373の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/2000. Detects a band of approximately 43 kDa.
Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa). |
特記事項 |
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WB
1/1000 - 1/2000. Detects a band of approximately 43 kDa. Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa). |
ターゲット情報
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関連性
Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation. -
細胞内局在
Type I membrane protein. -
参照データベース
- Entrez Gene: 1605 Human
- Entrez Gene: 13138 Mouse
- Omim: 128239 Human
- SwissProt: Q14118 Human
- SwissProt: Q62165 Mouse
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別名
- 156DAG antibody
- A3a antibody
- AGRNR antibody
see all
画像
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All lanes : Anti-beta Dystroglycan antibody [EP2200Y] (ab62373) at 1/500 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : DAG1 knockout HEK293T cell lysate
Lane 3 : Mouse heart tissue lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 43 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab62373 observed at 43 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab62373 Anti-beta Dystroglycan antibody [EP2200Y] was shown to specifically react with beta Dystroglycan in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266263 (knockout cell lysate ab257192) was used. Wild-type and beta Dystroglycan knockout samples were subjected to SDS-PAGE. ab62373 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-beta Dystroglycan antibody [EP2200Y] (ab62373) at 0.526 µg/ml (Purified)
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse skeletal muscle
Lane 4 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/mlBlocking Buffer: 5% NFDM /TBST
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Anti-beta Dystroglycan antibody [EP2200Y] (ab62373) at 1/1000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 43 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab62373 は論文での使用が確認できていません。