製品の概要

  • 製品名Anti-beta Defensin 3 antibody
    beta Defensin 3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to beta Defensin 3
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified recombinant full length human beta defensin 3.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab19270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 23627872

ターゲット情報

  • 機能Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistent E.faecium. No significant hemolytic activity was observed.
  • 組織特異性Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine.
  • 配列類似性Belongs to the beta-defensin family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BD 3 antibody
    • BD-3 antibody
    • BD3 antibody
    • beta 103 antibody
    • Beta defensin 103A antibody
    • Beta defensin 103A precursor antibody
    • Beta defensin 3 antibody
    • Beta-defensin 103 antibody
    • Beta-defensin 3 antibody
    • D103A_HUMAN antibody
    • DEF B3 antibody
    • DEFB 103 antibody
    • DEFB 103A antibody
    • DEFB 3 antibody
    • DEFB-3 antibody
    • DEFB103 antibody
    • DEFB103A antibody
    • DEFB103B antibody
    • DEFB3 antibody
    • Defensin antibody
    • Defensin beta 103A antibody
    • Defensin beta 3 antibody
    • Defensin like protein antibody
    • Defensin-like protein antibody
    • HBD 3 antibody
    • hBD-3 antibody
    • HBD3 antibody
    • HBP 3 antibody
    • HBP3 antibody
    • mBD3 antibody
    see all

Anti-beta Defensin 3 antibody 画像

  • ab19270 staining beta Defensin 3 in the A549 cell line from Human Lung by ICC/IF (Immunocytochemistry/immunofluorescence) treated with cigarette smoke extract. Cells were fixed , permeabilized and blocked. Samples were incubated with primary antibody (1/500) for 1 hour. An  Alexa Fluor®488-conjugated  was used as the secondary antibody and DAPI used to stain the nuclei.

Anti-beta Defensin 3 antibody (ab19270) 使用論文

This product has been referenced in:
  • Pierson T  et al. Cigarette smoke extract induces differential expression levels of beta-defensin peptides in human alveolar epithelial cells. Tob Induc Dis 11:10 (2013). ICC/IF ; Human . Read more (PubMed: 23627872) »
  • Maisetta G  et al. Evaluation of the inhibitory effects of human serum components on bactericidal activity of human beta defensin 3. Peptides 29:1-6 (2008). Read more (PubMed: 18045738) »

See all 2 Publications for this product

Product Wall

Sure, I am happy to send along the replacements.

If you could please remind me of the purchase order number or Abcam order reference number associated with the original purchase of these antibodies, I'll arrange replacements immediately.
Read More

Application Immunohistochemistry (Frozen sections)
Sample Equus Tissue sections (respiratory tract)
Permeabilization Yes - 0.1% Triton X 100
Specification respiratory tract
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Jolien Van Cleemput

Verified customer

投稿 Dec 07 2015

Does your customer want to use the same catalog numbers or would she be interested in choosing one or more different antibodies (of the same price) to the beta defensin proteins?

Thanks for passing along the peptide and protein concentration information.

These concentrations seem reasonable.

Is your customer interested in replacements for the antibodies?

Thank you for sending along the dot blots and additional information. We really appreciate the extra troubleshootingeffort.

Here are my thoughts:

1. In general it looks like the antibodies are able to detect Defensin in the chorioam...

Read More

Thank you for contacting Abcam Technical Team and for taking the time to provide some useful details of the experiments. I am very sorry to hear that your customer is are having problems with 8 of our products.  Special thanks for collecting the troubl...

Read More

Thank you for your enquiry. The immunogen was recombinant human beta-defensin-3. This same material was used in Western blots using an SDS-PAGE mini gel. I have updated the datasheet to reflect that the immunogen is recombinant. Please let us k...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"