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Our Abpromise guarantee covers the use of ab109571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution). ab109571, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human beta Defensin 1.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 7 kDa. Used in conjunction with compatible secondary reagents the detection limit for ab109571 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to beta Defensin 1 (Biotin) (ab84245). (using 100 µl/well antibody solution). ab109571, in conjunction with ab84245 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human beta Defensin 1.|
ab109571 has not yet been referenced specifically in any publications.