Anti-beta Catenin (phospho T41) 抗体 [BCT-41] (ab49825)

製品の概要

  • 製品名Anti-beta Catenin (phospho T41) antibody [BCT-41]
    beta Catenin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [BCT-41] to beta Catenin (phospho T41)
  • 特異性ab49825 reacts specifically with beta catenin phosphorylated at Thr41.
  • アプリケーション適用あり: WB, ELISA, ICC, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phospho-peptide corresponding to amino acids 38-50 (pThr41) of human beta-Catenin, conjugated to KLH.

  • ポジティブ・コントロール
    • Extract of cultured 293T (human embryonal kidney) cells treated with Lici (20 mM) and calyculin A (50 nM)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
ICC
IP
  • 追加情報ELISA: Use at an assay dependent dilution.
    ICC: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.

    WB: Use at a concentration of 8 - 10 µg/ml. Predicted molecular weight: 86 kDa.
    Note: Staining of the phospho beta catenin (pThr41) band in immunoblotting is specifically inhibited with the phosphorylated immunizing peptide (amino acids 38-45, pThr41) and not with the corresponding non phosphorylated peptide.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
      Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
    • 組織特異性Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
    • 関連疾患Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
      Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
      Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
      Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
      Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
      Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
    • 配列類似性Belongs to the beta-catenin family.
      Contains 12 ARM repeats.
    • 翻訳後修飾Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
      EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
      Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
    • 細胞内局在Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Beta catenin antibody
      • Beta-catenin antibody
      • Cadherin associated protein antibody
      • Catenin (cadherin associated protein), beta 1, 88kDa antibody
      • Catenin beta 1 antibody
      • Catenin beta-1 antibody
      • CATNB antibody
      • CHBCAT antibody
      • CTNB1_HUMAN antibody
      • CTNNB antibody
      • CTNNB1 antibody
      • DKFZp686D02253 antibody
      • FLJ25606 antibody
      • FLJ37923 antibody
      • OTTHUMP00000162082 antibody
      • OTTHUMP00000165222 antibody
      • OTTHUMP00000165223 antibody
      • OTTHUMP00000209288 antibody
      • OTTHUMP00000209289 antibody
      see all

    Anti-beta Catenin (phospho T41) antibody [BCT-41] 画像

    • HEK 293T cells, treated with LiCl (20 µg/mL), were labeled with 8ug/ml ab49825 Monoclonal Anti phospho-ß-Catenin (pThr41) followed by Goat Anti-Mouse IgG(Fab)-FITC conjugate.

    Anti-beta Catenin (phospho T41) antibody [BCT-41] (ab49825) 使用論文

    ab49825 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us.

    To our knowledge these products have not been tested on canine. However, based on a 99% sequence homology between human each should be viable on canine tissues. I have noted that you have taken advantage of our...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"