Anti-beta Catenin (phospho S33 + S37) 抗体 [BC-22] (ab11350)

製品の概要

  • 製品名
    Anti-beta Catenin (phospho S33 + S37) antibody [BC-22]
    beta Catenin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [BC-22] to beta Catenin (phospho S33 + S37)
  • 特異性
    Monoclonal Anti-phospho-beta-Catenin (pSer33/pSer37) reacts specifically with beta-catenin phosphorylated at Ser37, and Ser33. At the peptide level, the antibody is inhibited more efficiently by the pSer37 peptide than by the pSer33 peptide and is not inhibited by the nonphosphorylated peptide. The antibody does not detect the unphosphorylated or the Ser33 phosphorylated protein. It has a stronger affinity for phospho S37 than phospho S33. It does not recognize phosphorylated plakoglobin despite the high homology in the phosphorylation site with beta-catenin.
  • アプリケーション
    適用あり: WB, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Chicken, Human
  • 免疫原

    Synthetic peptide: DSp GIHSpGATTTAPS conjugated to KLH, corresponding to amino acids 32-45 of Human beta Catenin.

  • ポジティブ・コントロール
    • Whole extract of cultured 293 (human embryonal kidney) cells treated with the proteasome inhibitor MG132.
  • 特記事項
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 94 kDa. The peptide is highly phosphorylated in the cytosol, and thus is very unstable. When it is translocated to the nucleus, it is at a lower phosphorylation state, and is therefore more stable. You may be seeing degradation products of the protein, since it may be enzymatically degraded in the cytosol. A 50kDa form is present only if the proteosome is inhibited (by MG132 for example)
ICC/IF Use at an assay dependent concentration.
ELISA Use a concentration of 5 - 10 µg/ml.

ターゲット情報

  • 機能
    Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • 組織特異性
    Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • 関連疾患
    Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • 配列類似性
    Belongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • 翻訳後修飾
    Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • 細胞内局在
    Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

参考文献

This product has been referenced in:
  • Wang S  et al. Dissecting signaling pathways that govern self-renewal of rabbit embryonic stem cells. J Biol Chem 283:35929-40 (2008). WB ; Mouse, Rabbit . Read more (PubMed: 18940811) »

See 1 Publication for this product

レビューと Q&A

As product Anti-beta Catenin (phospho S33 + S37) antibody [BC-22] (ab11350) is designed for the detection of a phospho-target, we recommend use of BSA (rather than non-fat dry milk) as the blocking reagent for WB applications.

For additional i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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