Anti-beta Catenin (phospho S33 + S37) 抗体 (ab53050)

製品の概要

  • 製品名Anti-beta Catenin (phospho S33 + S37) antibody
    beta Catenin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to beta Catenin (phospho S33 + S37)
  • 特異性ab53050 detects endogenous levels of beta Catenin only when phosphorylated at serine 33 or serine 37.
  • アプリケーション適用あり: ELISA, IHC-P, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic phosphopeptide derived from human beta Catenin around the phosphorylation site of serine 33 or serine 37 (SP-G-I-H-SP).

  • ポジティブ・コントロール
    • Human colon carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 機能Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • 組織特異性Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • 関連疾患Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • 配列類似性Belongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • 翻訳後修飾Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

Anti-beta Catenin (phospho S33 + S37) antibody 画像

  • ab53050 staining beta Catenin in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with Triton-X and blocked with 10% serum for 1 hour at 20°C. The sample was incubated with primary antibody (1/100 in PBS + 10% Donkey serum + 0.1% Triton X-100) at 4°C for 16 hours. An undiltued Alexa Fluor® 568-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • ab53050 at 1/50 dilution staining beta Catenin in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue (in the absence or presence of the immunising peptide).ab53050 at 1/50 dilution staining beta Catenin in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue (in the absence or presence of the immunising peptide).

Anti-beta Catenin (phospho S33 + S37) antibody (ab53050) 使用論文

This product has been referenced in:

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - Triton-X 100
Fixative Paraformaldehyde
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Mr. Osama Aldalahmah

Verified customer

投稿 Aug 30 2013

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Unfortunately, because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of any of the antibodies requested. Read More

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To our knowledge these products have not been tested on canine. However, based on a 99% sequence homology between human each should be viable on canine tissues. I have noted that you have taken advantage of our...

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