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A synthetic phosphopeptide derived from human beta Catenin around the phosphorylation site of serine 33 or serine 37 (SP-G-I-H-SP).
Our Abpromise guarantee covers the use of ab53050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
ab53050 staining beta Catenin in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with Triton-X and blocked with 10% serum for 1 hour at 20°C. The sample was incubated with primary antibody (1/100 in PBS + 10% Donkey serum + 0.1% Triton X-100) at 4°C for 16 hours. An undiltued Alexa Fluor® 568-conjugated Donkey anti-rabbit polyclonal was used as the secondary antibody.