製品の概要

  • 製品名Anti-beta Catenin antibody [E247]
    beta Catenin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E247] to beta Catenin
  • アプリケーション適用あり: IHC-Fr, IHC-P, WB, ICC/IF, IPmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Sheep, Hamster, Cow, Human, Macaque Monkey, African Green Monkey
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human beta Catenin aa 1-100 (N terminal).

  • ポジティブ・コントロール
    • WB: A431 cells and lysate. IF: A431 cells. IHC-P: Human colon adenocarcinoma.
  • 特記事項

    A trial size is available to purchase for this antibody.

    Alternative versions available:

    Anti-beta Catenin antibody (BSA & Azide free) [E247] (ab196204)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/200.
IHC-P 1/500.
WB 1/5000 - 1/10000. Detects a band of approximately 92 kDa (predicted molecular weight: 86 kDa).
ICC/IF 1/250.
IP 1/100.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
      Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
    • 組織特異性Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
    • 関連疾患Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
      Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
      Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
      Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
      Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
      Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
    • 配列類似性Belongs to the beta-catenin family.
      Contains 12 ARM repeats.
    • 翻訳後修飾Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
      EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
      Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
    • 細胞内局在Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Beta catenin antibody
      • Beta-catenin antibody
      • Cadherin associated protein antibody
      • Catenin (cadherin associated protein), beta 1, 88kDa antibody
      • Catenin beta 1 antibody
      • Catenin beta-1 antibody
      • CATNB antibody
      • CHBCAT antibody
      • CTNB1_HUMAN antibody
      • CTNNB antibody
      • CTNNB1 antibody
      • DKFZp686D02253 antibody
      • FLJ25606 antibody
      • FLJ37923 antibody
      • OTTHUMP00000162082 antibody
      • OTTHUMP00000165222 antibody
      • OTTHUMP00000165223 antibody
      • OTTHUMP00000209288 antibody
      • OTTHUMP00000209289 antibody
      see all

    Anti-beta Catenin antibody [E247] 画像

    • ab32572 staining beta-catenin in the bEnd.5 murine cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton in PBS and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/300) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    • ab32572 staining beta Catenin in Dog colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in 10mM citrate buffer, pH6. Samples were incubated with primary antibody (1/250 in PBS with 1x casein) for 90 minutes at 25°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

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    • Performed under reducing conditions.

      Predicted band size : 86 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

      This image is courtesy of an anonymous Abreview

      Western blot image of ab32572 staining whole cell lysate of U2OS cells.  The gel was blocked with 5% milk for 1 hour at 21°C. The primary antibody was diluted 1/5000 and incubated  for 12 hours at 4°C.  A HRP conjugated swine anti-rabbit antibody was used as the secondary.

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    • ab32572 staining βcatenin in SW480 cells treated with BIO (ab120891), by ICC/IF. Increase of βcatenin expression correlates with increased concentration of BIO, as described in literature.
      The cells were incubated at 37°C for 48h in media containing different concentrations of ab120891 (BIO) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32572 (1/200) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
    •     ab32572 at 1/200 staining mouse small intestine tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step was performed before incubation with the primary antibody. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

      See Abreview

    • ab32572 staining beta Catenin in mouse liver tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocked with 5% serum at 40C for 30 minutes. The sample was incubated with primary antibody (1/200) in dilution buffer containing PBS and 3% Goat Serum at 40C for 9 hours. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.

      See Abreview

    • ab32572 staining human renal carcinoma tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking with 1% milk for 45 minutes at 22°C.  The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 22°C.  A HRP conjugated goat anti-rabbit antibody, diluted 1/400, was used as the secondary.

      See Abreview

    • ab32572 staining β catenin in SKNSH cells treated with olanzapine (ab120736), by ICC/IF. Increase in expression of β catenin correlates with increased concentration of olanzapine, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120736 (olanzapine) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32572 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
    • ab32572 showing positive staining in Cervical carcinoma tissue.

    • ab32572 showing positive staining in Breast carcinoma tissue.

    • ab32572 showing positive staining in Lung adenocarcinoma tissue.

    • ab32572 showing positive staining in Papillary carcinoma of thyroid gland tissue.

    • ab32572 showing positive staining in Kidney carcinoma tissue.

    Anti-beta Catenin antibody [E247] (ab32572) 使用論文

    This product has been referenced in:

    See all 50 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
    Sample Mouse Cell (mouse Embryonic stem cell)
    Specification mouse Embryonic stem cell
    Permeabilization Yes - 0.1% triton X
    Fixative Methanol:acetone
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    投稿 Jul 08 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA+Goat IgG as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Sample Human Cell (Breast epithelial)
    Specification Breast epithelial
    Permeabilization Yes - Triton X-100
    Fixative Paraformaldehyde
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    投稿 Jun 04 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Cow Tissue sections (oviductal tissue)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
    Permeabilization No
    Specification oviductal tissue
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative Paraformaldehyde
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    投稿 Sep 28 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Pig Tissue sections (Mammary epithelial cells)
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: 10mM citrate buffer
    Permeabilization No
    Specification Mammary epithelial cells
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative Formaldehyde
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    Mrs. Katie Elliott

    Verified customer

    投稿 Aug 15 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (human breast cancer cells)
    Gel Running Conditions Reduced Denaturing (Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels, 10-well, 50 µl)
    Loading amount 80 µg
    Specification human breast cancer cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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    Mrs. Katie Elliott

    Verified customer

    投稿 Aug 01 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Zebra finch Tissue sections (brain)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
    Permeabilization No
    Specification brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Formaldehyde
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    投稿 Jun 24 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (C2C12 Cells)
    Gel Running Conditions Reduced Denaturing (4-15)
    Loading amount 40 µg
    Specification C2C12 Cells
    Blocking step Sea Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
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    投稿 Jun 06 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Penguin Tissue sections (brain)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
    Permeabilization No
    Specification brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative Formaldehyde
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    Abcam user community

    Verified customer

    投稿 May 03 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Finch (Fringillidae) Tissue sections (brain)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
    Permeabilization No
    Specification brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative Formaldehyde
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    投稿 Mar 23 2016

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Horse Tissue sections (aglandular stomach, epithelium)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization No
    Specification aglandular stomach, epithelium
    Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative Methacarn
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    Dr. F Hernandez-Blazquez

    Verified customer

    投稿 Mar 17 2016

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