製品の概要

  • 製品名Anti-beta Catenin antibody
    beta Catenin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to beta Catenin
  • 特異性Reacts in dot blot with beta-catenin peptide 768-781 conjugated to BSA. In immunoblots, reacts with a 94kD protein in extracts of Madin-Darby Bovine Kidney (MDBK) cultured cells. Specific staining is inhibited following pre-incubation of the antiserum with the beta-catenin peptide. Shows no reactivity with BSA conjugated alpha catenin peptide (amino acids 890-901). The antibody does not cross react with a-catenin or ?-catenin (plakoglobin).
  • アプリケーション適用あり: ICC/IF, Dot blot, IHC-Fr, WB, IHC-P, IHC - Wholemountmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Horse, Chicken, Dog, Human, Pig, Xenopus laevis, Zebrafish, Botryllus schlosseri
  • 免疫原

    Synthetic peptide:

    PGDSNQLAWFDTDL

    conjugated to KLH, corresponding to amino acids 768-781 of Human or mouse ß Catenin. (Peptide available as ab16377)

  • ポジティブ・コントロール
    • MDBK cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/2000. PubMed: 18624906
Dot blot 1/4000.
IHC-Fr 1/2000.
WB 1/4000. Predicted molecular weight: 94 kDa.
IHC-P Use at an assay dependent concentration.
IHC - Wholemount 1/200.

ターゲット情報

  • 機能Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
    Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.
  • 組織特異性Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
  • 関連疾患Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
    Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
    Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
    Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
    Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
    Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • 配列類似性Belongs to the beta-catenin family.
    Contains 12 ARM repeats.
  • 翻訳後修飾Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
    EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
    Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation.
  • 細胞内局在Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta catenin antibody
    • Beta-catenin antibody
    • Cadherin associated protein antibody
    • Catenin (cadherin associated protein), beta 1, 88kDa antibody
    • Catenin beta 1 antibody
    • Catenin beta-1 antibody
    • CATNB antibody
    • CHBCAT antibody
    • CTNB1_HUMAN antibody
    • CTNNB antibody
    • CTNNB1 antibody
    • DKFZp686D02253 antibody
    • FLJ25606 antibody
    • FLJ37923 antibody
    • OTTHUMP00000162082 antibody
    • OTTHUMP00000165222 antibody
    • OTTHUMP00000165223 antibody
    • OTTHUMP00000209288 antibody
    • OTTHUMP00000209289 antibody
    see all

Anti-beta Catenin antibody 画像

  • All lanes : Anti-beta Catenin antibody (ab6302) at 1/4000 dilution

    Lane 1 : 5ug human lung tumour lysate.
    Lane 2 : 10ug human lung tumour lysate.
    Lane 3 : 20ug human lung tumour lysate.

    Secondary
    Goat anti-Rabbit IgG (H&L)HRP

    Performed under reducing conditions.

    Predicted band size : 94 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)


    Exposure time : 10 seconds
    This image is courtesy of an Abreview submitted by Mike Campa on 4 April 2006.

    See Abreview

  • Immunohistochemical staining of paraffin embedded, PFA fixed, mouse liver using ab6302 at 1/200. Heat mediated antigen retrieval was performed using sodium citrate buffer, pH 6, and the sample was permeabilized with 0.05% Triton X 100. The sample was blocked in 5% BSA for 1 hour at room temperature and was then incubated with primary antibody for 16 hours in PBS/0.05% Triton X 100 at 4 °C. An Alexa Fluor® 488 donkey anyti-rabbit was used as the secondary at 1/250.

    See Abreview

  • IHC-Wholemount image of anti-beta Catenin antibody (ab6302) staining on the Zebrafish jaw of a 5 day old larva. This larva was stained with anti-beta Catenin antibody (green) and a collagen 2 antibody (red) for 2 hours at 20°C. The image shows the cartilage elements of the jaw.

    See Abreview

  • This picture was kindly supplied as part of the review submitted by Mohaiza Dashwood. Immunofluorescence of H29 cells stained with DAPI (blue) and rabbit polyclonal anti-beta-catenin (ab6302), 1/2000) with Alexa Fluor 488 (green) from Molecular Probes.

     

  • ab6302 at 1/2000 dilution staining beta Catenin in human HeLa cells by immunocytochemistry/ immunofluorescence. Sections were paraformaldehyde fixed, permeabilized in 0.3% Triton X prior to blocking in 5% BSA for 2 hours at 27°C and then incubated with ab6302 for 8 hour at 4°C. Alexa fluor® 488 goat polyclonal, diluted 1/1000, was used as the secondary antibody. Counterstaining with DAPI.

    See Abreview

  • ab6302 at 1/500 dilution staining beta Catenin in human breast cells by immunocytochemistry/ immunofluorescence. Sections were formaldehyde fixed, permeabilized in 0.5% digitonin prior to incubating with ab6302 for 1 hour. Alexa fluor® 488 goat polyclonal, diluted 1/500, was used as the secondary antibody. Treated samples recieved 10um GSK-3 Inhibitor X.

    See Abreview

Anti-beta Catenin antibody (ab6302) 使用論文

This product has been referenced in:
  • Hwang PY  et al. N-cadherin is Key to Expression of the Nucleus Pulposus Cell Phenotype under Selective Substrate Culture Conditions. Sci Rep 6:28038 (2016). IHC-P, ICC/IF . Read more (PubMed: 27292569) »
  • Quist SR  et al. Expression of epidermal stem cell markers in skin and adnexal malignancies. Br J Dermatol 175:520-30 (2016). Read more (PubMed: 26914519) »

See all 41 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (RLE-6TN)
Permeabilization Yes - 0.1% triton
Specification RLE-6TN
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative Formaldehyde
Username

Yuchin

Verified customer

投稿 Aug 29 2016

As stated on the datasheet for ab6302, the purity of this antibody is whole antiserum. Thus, there are other proteins and IgGs present that make a concentration measurement of the specific antibody itself inaccurate, so the total protein concentration ...

Read More
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 4°C
Sample Human Cell (Human arterial myocytes)
Specification Human arterial myocytes
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Apr 08 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Na-citrate pH6
Sample Mouse Tissue sections (Liver, P5)
Specification Liver, P5
Permeabilization Yes - Triton 0,05%
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 29 2014

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application IHC - Wholemount
Sample Zebrafish Embryo (Whole mount 3, 4 and 5 day old larvae)
Specification Whole mount 3, 4 and 5 day old larvae
Username

Abcam user community

Verified customer

投稿 Jun 06 2013

Thank you for contacting us.

Unfortunately, because we carry over 100,000 products, it isn't feasible for us to keep small sample sizes of our products, and therefore I am not able to offer a test sample of any of the antibodies requested. Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Sheep Tissue sections (Sheep tibial plateau articular cartilage and bone)
Specification Sheep tibial plateau articular cartilage and bone
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step DAKO protein block-serum free (X0909) as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jan 31 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (SW1353 treated with rhWnt3A for 24 hours)
Specification SW1353 treated with rhWnt3A for 24 hours
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 31 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (SW1353)
Loading amount 20 µg
Specification SW1353
Treatment 50ng/mL rhWnt3A
Gel Running Conditions Reduced Denaturing (10% Bis-Tris Gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jan 30 2013

Merci de nous avoir contactés.

La séquence immunogène d’ab6302 est xxxxxxxxxxx qui correspond aux acides aminés xxxxxxxx de la région C-terminale de la protéine chez l’humain. Cependant, c...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"