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Our Abpromise guarantee covers the use of ab23671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21317240|
|WB||Use at an assay dependent concentration. Detects a band of approximately 96 kDa (predicted molecular weight: 86-97 kDa).
Sufficient for 10 western blots.
|IHC-P||Use a concentration of 1 - 2 µg/ml.|
Immunocytochemistry/ Immunofluorescence analysis of mouse EpH4.9 cells labeling beta Catenin with ab23671 (green). Cells were fixed in 4% paraformaldehyde and incubated with Anti-beta Catenin antibody (ab23671), followed by an appropriate secondary antibody. The actin cytoskeleton was stained with phalloidin and cell nuclei with DAPI. Immunofluorescence images were obtained using confocal microscopy and Imaris imaging software.
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