製品の概要
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製品名Anti-beta Catenin antibody
beta Catenin 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to beta Catenin
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アプリケーション適用あり: IP, IHC-P, ICC/IF, IHC-FoFr, Sandwich ELISA, WBmore details
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種交差性交差種: Mouse, Rat, Sheep, Goat, Cat, Dog, Human, Pig, Xenopus laevis, Zebrafish, Common marmoset, Squirrel, Dogfish, Catshark
交差が予測される動物種: Chicken
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human beta Catenin.
(Peptide available as ab16377.)
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ポジティブ･コントロール
- ab16051 gave a positive result in the following whole cell lysates: Hela, A431 ab16051 gave a positive result in the following tissue lysates: Mouse Brain Mouse Testis Mouse Spinal Cord Mouse Ovary Rat Brain ab16051 gave a positive result in the following FFPE tissue; Human colon adenocarcinoma, human liver normal.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Immunohistochemistry kits
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab16051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use at an assay dependent concentration. PubMed: 24614104 | |
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 µg/ml. | |
| IHC-FoFr | 1/100. | |
| Sandwich ELISA | Use a concentration of 0.1 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.1 µg/ml with Mouse monoclonal [BDI080] to beta Catenin (ab19448) as Capture. | |
| WB | Use a concentration of 0.25 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 94 kDa).Can be blocked with Human beta Catenin peptide (ab16377). |
ターゲット情報
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機能Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. -
組織特異性Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon.
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関連疾患Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1. -
配列類似性Belongs to the beta-catenin family.
Contains 12 ARM repeats. -
翻訳後修飾Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation. -
細胞内局在Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.
- Information by UniProt
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参照データベース
- Entrez Gene: 395964 Chicken
- Entrez Gene: 1499 Human
- Entrez Gene: 12387 Mouse
- Entrez Gene: 397657 Pig
- Entrez Gene: 84353 Rat
- Entrez Gene: 30265 Zebrafish
- Omim: 116806 Human
- SwissProt: P35222 Human
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別名
- Beta catenin antibody
- Beta-catenin antibody
- Cadherin associated protein antibody
see all
Anti-beta Catenin antibody 画像
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Western blot - Anti-beta Catenin antibody (ab16051)
Predicted band size : 94 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: CTNNB1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: A431 whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab16051 observed at 95 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab16051 was shown to recognize CTNNB1 when CTNNB1 knockout samples were used, along with additional cross-reactive bands. Wild-type and CTNNB1 knockout samples were subjected to SDS-PAGE. Ab16051 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 0.25 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)ab16051 staining β-Catenin in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab16051 at 1μg/ml and ab7291 (staining Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Mouse secondary (ab150120) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)IHC image of beta catenin staining in a formalin fixed, paraffin embedded normal human liver tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16051, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)IHC image of beta catenon staining in a formalin fixed, paraffin embedded human colon adenocarcinamo tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16051, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab16051)This image is courtesy of an Abreview by Carl Hobbs.ab16051 staining beta Catenin in rat brain tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
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Western blot - Anti-beta Catenin antibody (ab16051)All lanes : Anti-beta Catenin antibody (ab16051) at 0.25 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa whole cell lysate withHuman beta Catenin peptide (ab16377) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 94 kDa
Observed band size : 94 kDa
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.A second band of 75 kDa was also detected in Hela whole cell lysates and A431 lysates. This smaller band was of equal intensity to the 94 kDa band in the A431 lysates (data not shown).
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Western blot - Anti-beta Catenin antibody (ab16051)Anti-beta Catenin antibody (ab16051) at 0.25 µg/ml +Recombinant Human beta Catenin protein (ab63175) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 94 kDa
Exposure time : 10 seconds -
Sandwich ELISA - Anti-beta Catenin antibody (ab16051)Standard Curve for Beta-Catenin (Analyte: beta Catenin protein (Tagged) (ab63175)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [BDI080] to beta Catenin (ab19448) at 1ug/ml and Detector Antibody Rabbit polyclonal to beta Catenin (ab16051) at 0.1ug/ml. -
Immunohistochemistry paraffin embedded sections - Anti-beta Catenin antibody (ab16051)This image is courtesy of an Abreview by Carl Hobbs.ab16051 staining beta Catenin in developing gut tissue sections from mouse by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 2 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)ab16051 staining β-catenin in SW480 cells treated with XAV939 (ab120897), by ICC/IF. Increase of ß-catenin cytoplasmic expression and decrease in nuclear expression correlates with increased concentration of XAV939, as described in literature.
The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab120897 (XAV939) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab16051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)ICC/IF image of ab16051 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16051X, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Immunocytochemistry/ Immunofluorescence - Anti-beta Catenin antibody (ab16051)This image is courtesy of an Abreview submitted by Miss Helen Gillinghamab16051 staining human fibrosarcoma cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton X-100 prior to incubation with the primary antibody (at 10µg/ml) for 1 hour at 27°C. A Texas Red® conjugated donkey anti-rabbit antibody was used as the secondary.
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-beta Catenin antibody (ab16051)This image is a courtesy of Sophie Pezetab16051 staining beta Catenin in rat hypothalamus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat. The sample was incubated with primary antibody (1/100) for 18 hours at 200C in PBS + 0.3 % Triton X100. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/1000 dilution.
プロトコール
Anti-beta Catenin antibody (ab16051) 使用論文
This product has been referenced in:
- Kong LY et al. In vivo and in vitro effects of microRNA-27a on proliferation, migration and invasion of breast cancer cells through targeting of SFRP1 gene via Wnt/ß-catenin signaling pathway. Oncotarget 8:15507-15519 (2017). WB ; Human . Read more (PubMed: 28099945) »
- Zhang X et al. Dickkopf-related protein 3 negatively regulates the osteogenic differentiation of rat dental follicle cells. Mol Med Rep 15:1673-1681 (2017). WB ; Rat . Read more (PubMed: 28259940) »
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