製品の概要

  • 製品名Anti-beta Bax antibody
    beta Bax 一次抗体 製品一覧
  • 製品の詳細
    Sheep polyclonal to beta Bax
  • アプリケーション適用あり: IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide:

    LTTAPAPPSLPPATPLGPWAFWSRSQWC

    , corresponding to amino acids 172-199 of Human beta Bax.

  • ポジティブ・コントロール
    • in vitro translated Bax beta

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP
  • 追加情報
    IP: Use at an assay dependent dilution.

    Is unsuitable for WB. Will not detect denatured protein.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
    • 組織特異性Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
    • 配列類似性Belongs to the Bcl-2 family.
    • ドメインIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
    • 細胞内局在Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Apoptosis regulator BAX antibody
      • Apoptosis regulator Bax cytoplasmic isoform beta antibody
      • BAX antibody
      • Bax B antibody
      • Bax beta antibody
      • BAX_HUMAN antibody
      • Bcl-2-like protein 4 antibody
      • BCL2 associated X protein antibody
      • BCL2 associated X protein isoform beta antibody
      • Bcl2-L-4 antibody
      see all

    Anti-beta Bax antibody 画像

    • Immunoprecipitation of in vitro translated Bax alpha (1) and Bax beta (2) using Bax antibody clone 6A7, Bax alpha native (3) and denatured protein(4) using Bax beta antibody ab16143, and Bax beta native (5) and denatured (6) protein using Bax beta antibody ab16143. Ab16143 immunoprecipitated Bax beta strongly however did not detect Bax beta by western blotting.

    Anti-beta Bax antibody (ab16143) 使用論文

    ab16143 has not yet been referenced specifically in any publications.

    Product Wall

    I was informed that this antibody was not tested in house and therefore we unfortunately do not have an in house protocol for it. I would recommend the following general IP protocol which should work with ab16143 and can be found on our website: ...

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    The antibody cannot detect the protein by western when the protein has been reduced because the reduction process opens up the protein which was in 3D and it becomes all unraveled. Some antibodies recognise an epitope which is a 3D part and therefore o...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"