Anti-beta B1 Crystallin 抗体 (ab54655)


  • 製品名Anti-beta B1 Crystallin antibody
    beta B1 Crystallin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to beta B1 Crystallin
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human, Recombinant fragment
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-253 of Human beta B1 Crystallin


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野


Our Abpromise guarantee covers the use of ab54655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Crystallins are the dominant structural components of the vertebrate eye lens.
    • 関連疾患Defects in CRYBB1 are the cause of cataract congenital nuclear autosomal recessive type 3 (CATCN3) [MIM:611544]. A congenital cataract affecting the central nucleus of the eye. Nuclear cataracts are often not highly visually significant. The density of the opacities varies greatly from fine dots to a dense, white and chalk-like, central cataract. The condition is usually bilateral. Nuclear cataracts are often combined with opacified cortical fibers encircling the nuclear opacity, which are referred to as cortical riders.
      Defects in CRYBB1 are a cause of cataract-microcornea syndrome (CAMIS) [MIM:116150]. An ocular disorder characterized by the association of congenital cataract and microcornea without any other systemic anomaly or dysmorphism. Clinical findings include a corneal diameter inferior to 10 mm in both meridians in an otherwise normal eye, and an inherited cataract, which is most often bilateral posterior polar with opacification in the lens periphery. The cataract progresses to form a total cataract after visual maturity has been achieved, requiring cataract extraction in the first to third decade of life. Microcornea-cataract syndrome can be associated with other rare ocular manifestations, including myopia, iris coloboma, sclerocornea and Peters anomaly. Transmission is in most cases autosomal dominant, but cases of autosomal recessive transmission have recently been described.
    • 配列類似性Belongs to the beta/gamma-crystallin family.
      Contains 4 beta/gamma crystallin 'Greek key' domains.
    • ドメインHas a two-domain beta-structure, folded into four very similar Greek key motifs.
    • 翻訳後修飾Specific cleavages in the N-terminal arm occur during lens maturation and give rise to truncated forms, leading to impaired oligomerization and protein insolubilization.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Beta crystallin B1 antibody
      • Beta-B1 crystallin antibody
      • Beta-crystallin B1 antibody
      • CATCN3 antibody
      • CRBB1_HUMAN antibody
      • CRYBB 1 antibody
      • Crybb1 antibody
      • Crystallin beta B1 antibody
      • CTRCT17 antibody
      • Eye lens structural protein antibody
      • OTTHUMP00000028719 antibody
      see all

    Anti-beta B1 Crystallin antibody 画像

    • Western blot against tagged recombinant protein immunogen using ab54655 beta B1 Crystallin antibody at 1ug/ml. Predicted band size of immunogen is 54 kDa.

      This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    • All lanes : Anti-beta B1 Crystallin antibody (ab54655) at 1/100 dilution

      Lane 1 : Zebrafish lens homogenate
      Lane 2 : Zebrafish lens homogenate
      Lane 4 : Human Beta B1 crystallin
      Lane 5 : Human Beta B2 crystallin (Negative Control)

      Lysates/proteins at 1 µg per lane.

      Alkaline phosphatase-conjugated Goat anti-mouse IgG monoclonal at 1/3000 dilution

      Observed band size : 45 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 30 kDa,82 kDa. We are unsure as to the identity of these extra bands.

      This image is courtesy of an Abreview submitted by Dr. Leonel Trujillo

      See Abreview

    Anti-beta B1 Crystallin antibody (ab54655) 使用論文

    ab54655 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Recombinant protein (lens)
    Loading amount 1 µg
    Specification lens
    Gel Running Conditions Non-reduced Denaturing (10%)
    Blocking step SuperBlock Blocking Buffer-Blotting in TBS as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C

    Leonel Trujillo

    Verified customer

    投稿 Apr 15 2010