製品の概要

  • 製品名Anti-beta Actin antibody [SP124]
    beta Actin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP124] to beta Actin
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide near the N-terminus of Human beta Actin.

  • ポジティブ・コントロール
    • Raji cell lystate, Human Placenta tissue

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab115777 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200. Predicted molecular weight: 42 kDa.
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

Anti-beta Actin antibody [SP124] 画像

  • Flow cytometric analysis of rabbit anti-beta Actin (SP124) antibody ab115777 (1/100) in HeLa cells (green) compared to negative control of rabbit IgG (blue)

  • Anti-beta Actin antibody [SP124] (ab115777) at 1/200 dilution + Raji cell lystate

    Predicted band size : 42 kDa
  • Staining of Human beta Actin in a Formalin/PFA-fixed paraffin-embedded section of Human Placenta using ab115777 at a dilution of 1/200.

Anti-beta Actin antibody [SP124] (ab115777) 使用論文

This product has been referenced in:
  • Cai SD  et al. MicroRNA-144 inhibits migration and proliferation in rectal cancer by downregulating ROCK-1. Mol Med Rep 12:7396-402 (2015). Read more (PubMed: 26458302) »
  • Navarro-Tableros V  et al. Recellularization of rat liver scaffolds by human liver stem cells. Tissue Eng Part A 21:1929-39 (2015). IHC-Fr ; Human . Read more (PubMed: 25794768) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"