The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).Can be blocked with Human beta Actin peptide (ab13772). Abcam strongly recommends blocking and incubation using 5% BSA as we have found that use of 5% milk seems to drastically reduce the intensity of the signal obtained. Please refer to WB image for further details.
機能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
関連疾患Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
Baran-Gale J et al. An integrative transcriptomics approach identifies miR-503 as a candidate master regulator of the estrogen response in MCF-7 breast cancer cells. RNA22:1592-603 (2016).
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Fan C et al. Zbed3 contributes to malignant phenotype of lung cancer via regulating ß-catenin and P120-catenin 1. Mol Carcinog54 Suppl 1:E138-47 (2015).
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