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Synthetic peptide derived from residues 1 - 100 of Human beta Actin.
Our Abpromise guarantee covers the use of ab13822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa).Can be blocked with Human beta Actin peptide (ab13772).|
IHC image of beta Actin staining in THuman normal colon formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab13822, 10µg/ml overnight at +4°C. A goat-anti chicken HRP secondary antibody (Ab6877, 1/500 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre