Anti-beta Actin 抗体 [AC-15] (FITC) (ab6277)

製品の概要

  • 製品名Anti-beta Actin antibody [AC-15] (FITC)
    beta Actin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AC-15] to beta Actin (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • アプリケーション適用あり: ICC/IF, ICC, IHC-Fr, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig, Drosophila melanogaster, Fish
  • 免疫原

    Synthetic peptide:

    DDDIAALVIDNGSGL

    conjugated to KLH, corresponding to amino acids 1-14 of Xenopus laevis Actin (beta).

  • エピトープThe N-terminal of the beta isoform of actin
  • ポジティブ・コントロール
    • Cultured human or chicken fibroblasts. Molecular weight of beta actin is 42Kd.
  • 特記事項Immunofluoresence staining of chicken gizzard ultra-thin sections labels the dense bodies, longitudinal channels and membrane associated dense-plaque.


    Useful for direct immunofluoresence labelling of the cytoskeleton in cultured cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent concentration. PubMed: 21562077
IHC-FoFr Use at an assay dependent dilution. PubMed: 21445349

ターゲット情報

Anti-beta Actin antibody [AC-15] (FITC) 画像

  • ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence. 

    30 min blocking with TBS-T / 5% BSA
    1 hr incubation with ab6277 (TBS-T / 5% BSA)
    1 minute nuclear counterstaining with DAPI

    Magnification x20

  • ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.

    30 min blocking with TBS-T / 5% BSA
    1 hr incubation with ab6277 (TBS-T / 5% BSA)
    1 minute nuclear counterstaining with DAPI

    Magnification x40 no deconvolution.

  • ab6277 at 1/100 dilution staining beta Actin in rat brain endothelial cells by Immunofluorescence.

    30 min blocking with TBS-T / 5% BSA
    1 hr incubation with ab6277 (TBS-T / 5% BSA)
    1 minute nuclear counterstaining with DAPI

    Magnification x40  “Inverse Filter” Deconvolution.

プロトコール

Anti-beta Actin antibody [AC-15] (FITC) (ab6277) 使用論文

This product has been referenced in:
  • Llewellyn KJ  et al. A Fine Balance of Dietary Lipids Improves Pathology of a Murine Model of VCP-Associated Multisystem Proteinopathy. PLoS One 10:e0131995 (2015). Read more (PubMed: 26134519) »
  • Perrin BJ  et al. ß-Actin and fascin-2 cooperate to maintain stereocilia length. J Neurosci 33:8114-21 (2013). Mouse . Read more (PubMed: 23658152) »

See all 10 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (neuroglioma cells)
Gel Running Conditions Reduced Denaturing (12%Acrylamide SDSPAGE)
Loading amount 100 µg
Treatment 0.5%triton
Specification neuroglioma cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Ms. Samira Acajjaoui

Verified customer

投稿 Aug 29 2016

Thank you for your reply. While we do not generally give discounts for teaching institutions in particular, if you are starting a new lab, moving a lab, or a first-time grant recipient, you may qualify for our New Lab Discount Program:

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Thank you for contacting us today. I can confirm that that these images were labelled incorrectly and have been updated. ab6277 was used in all 3 images. These changes should be visible on the PWS but can take a couple of days to be refreshed. ...

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Unfortunately we are unable to provide a concentration for this antibody because it is conjugated to FITC.

This antibody has not yet been tested for use with on formalin fixed, paraffin sections of mouse tissue.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"