製品の概要

  • 製品名Anti-beta Actin antibody [8H10D10]
    beta Actin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8H10D10] to beta Actin
  • アプリケーション適用あり: WB, ICC/IF, Flow Cyt, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Hamster, Human, Monkey
  • 免疫原

    Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.

  • ポジティブ・コントロール
    • NIH/3T3, Jurkat, Hela, CHO, PC12, HEK293, COS, A549 or MCF-7 cell lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab101173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 42 kDa).
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ELISA 1/10000.

ターゲット情報

  • 機能Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
  • 関連疾患Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
  • 配列類似性Belongs to the actin family.
  • 翻訳後修飾ISGylated.
  • 細胞内局在Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A26C1A antibody
    • A26C1B antibody
    • ACTB antibody
    • ACTB_HUMAN antibody
    • Actin cytoplasmic 1 antibody
    • Actin, cytoplasmic 1, N-terminally processed antibody
    • Actx antibody
    • b actin antibody
    • Beta cytoskeletal actin antibody
    • Beta-actin antibody
    • BRWS1 antibody
    • E430023M04Rik antibody
    • MGC128179 antibody
    • PS1TP5 binding protein 1 antibody
    • PS1TP5BP1 antibody
    see all

Anti-beta Actin antibody [8H10D10] 画像

  • All lanes : Anti-beta Actin antibody [8H10D10] (ab101173)

    Lane 1 : NIH/3T3
    Lane 2 : Jurkat
    Lane 3 : HeLa
    Lane 4 : CHO
    Lane 5 : PC12
    Lane 6 : HEK293
    Lane 7 : COS
    Lane 8 : A549
    Lane 9 : MCF-7


    Predicted band size : 42 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
  • ab101173 staining beta Actin in SKBR-3 (left) and A549 (right) cells by Immunocytochemistry/Immunofluorescence. A Cy3-conjugated Goat polyclonal to Mouse IgG was used as the secondary antibody. Nuclei were stained blue with DRAQ5 DNA fluorescent dye.
  • ab101173 staining beta Actin in MCF-7 cells (right) against staining using a negative control (left)

Anti-beta Actin antibody [8H10D10] (ab101173) 使用論文

This product has been referenced in:
  • Venza M  et al. miR-92a-3p and MYCBP2 are involved in MS-275-induced and c-myc-mediated TRAIL-sensitivity in melanoma cells. Int Immunopharmacol 40:235-243 (2016). Read more (PubMed: 27620505) »
  • Huang L  et al. Potential pitfalls and solutions for use of fluorescent fusion proteins to study the lysosome. PLoS One 9:e88893 (2014). WB . Read more (PubMed: 24586430) »

See all 3 Publications for this product

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There are currently no Abreviews or Questions for ab101173.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"