製品の概要

  • 製品名Anti-beta Actin antibody [4C2]
    beta Actin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4C2] to beta Actin
  • 特異性The epitope recognized by 4C2 is highly conserved and the antibody is therefore expected to react with many species. ab123020 does not cross react with other actin isoforms.
  • アプリケーション適用あり: WB, ELISA, IHC-P, IHC-Fr, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Chicken, Human, Pig
    交差が予測される動物種: a wide range of other species非交差種: Zebrafish
  • 免疫原

    Synthetic peptide:

    Ac-DDDIAALVV-COOH

    conjugated to KLH through the cysteine residue, corresponding to N terminal amino acids 2-10 of Human beta Actin.

  • ポジティブ・コントロール
    • Human dermal fibroblast cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab123020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 42 kDa.
ELISA Use at an assay dependent concentration.
IHC-P 1/100 - 1/500.
IHC-Fr 1/100 - 1/500.
Flow Cyt Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.

ターゲット情報

Anti-beta Actin antibody [4C2] 画像

  • Overlay histogram showing HeLa cells stained with ab123020 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab123020, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • ab123020 at 1/500 dilution staining beta Actin in methanol-fixed Human dermal fibroblasts by Immunofluorescence.
  • Immunofluorescence staining of 3 days old zebrafish embryo
  • Immunofluorescence staining of 1 month old zebrafish embryo

Anti-beta Actin antibody [4C2] (ab123020) 使用論文

This product has been referenced in:
  • Scott DW  et al. Heterogenic endothelial responses to inflammation: role for differential N-glycosylation and vascular bed of origin. J Am Heart Assoc 2:e000263 (2013). WB ; Human . Read more (PubMed: 23900214) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab123020.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"