Application
Western blot
Sample
Xenopus laevis Tissue lysate - whole (Head lysates)
Loading amount
30 µg
Specification
Head lysates
Gel Running Conditions
Reduced Denaturing (12% TGX (Bio-Rad))
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/5000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.5% milk/TBST
Secondary antibody
Name
Non-Abcam antibody was used: Signalchem Anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL Prime
Exposure
1 second(s)
Bands
Specific: 52 kDa
Additional data
Additional Notes
The signal was very strong - the concentration of the antibody can be reduced much further.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 19 2012