HRP Anti-beta IV Tubulin 抗体 [ONS.1A6] (ab204454)
Key features and details
- HRP Mouse monoclonal [ONS.1A6] to beta IV Tubulin
- Suitable for: WB
- Reacts with: Mouse, Human
- Conjugation: HRP
- Isotype: IgG1
Related conjugates and formulations
製品の概要
-
製品名
HRP Anti-beta IV Tubulin antibody [ONS.1A6]
beta IV Tubulin 一次抗体 製品一覧 -
製品の詳細
HRP Mouse monoclonal [ONS.1A6] to beta IV Tubulin -
由来種
Mouse -
標識
HRP -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Hamster, Cow -
免疫原
Synthetic peptide corresponding to beta IV Tubulin (C terminal) conjugated to bovine serum albumin.
-
ポジティブ・コントロール
- WB: HeLa, HEK293 and NIH3T3 whole cell lysates.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
-
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
ONS.1A6 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Alternative Versions
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab204454の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa).
|
特記事項 |
---|
WB
1/5000. Detects a band of approximately 52 kDa (predicted molecular weight: 50 kDa). |
ターゲット情報
-
機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. -
配列類似性
Belongs to the tubulin family. -
ドメイン
The highly acidic C-terminal region may bind cations such as calcium. -
翻訳後修飾
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
-
参照データベース
- Entrez Gene: 540236 Cow
- Entrez Gene: 10382 Human
- Entrez Gene: 22153 Mouse
- Entrez Gene: 29213 Rat
- Omim: 602662 Human
- SwissProt: Q3ZBU7 Cow
- SwissProt: P04350 Human
- SwissProt: Q9D6F9 Mouse
see all -
別名
- Beta 4 antibody
- Beta 4 tubulin antibody
- beta 5 antibody
see all
画像
-
All lanes : HRP Anti-beta IV Tubulin antibody [ONS.1A6] (ab204454) at 1/5000 dilution
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204454 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab204454 は論文での使用が確認できていません。