Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Penguin Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS plus 1xcasein
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Immpress universal
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
no cross reaction to this antibody, while GFAP and NF200 are strongly positive as review submitted earlier
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 01 2016