beta Galactosidase Staining Kit (ab102534)
Key features and details
- Assay type: Direct
- Sample type: Adherent cells, Suspension cells
製品の概要
-
製品名
beta Galactosidase Staining Kit
beta galactosidase キット 製品一覧 -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Direct -
製品の概要
The LacZ gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of promoters of genes. The LacZ gene encodes the enzyme ß-galactosidase, which is very stable, resistant to proteolytic degradation, can utilize a variety of substrates and can be easily assayed in situ.
Abcam's beta Galactosidase Detection Kit (ab102534) utilizes X-gal as the substrate for ß-galactosidase.
Visit our FAQs page for tips and troubleshooting.
-
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K802 Beta-Galactosidase Staining Kit. K802-250 is the same size as the 250 test size of ab102534.
This kit has been optimized for transfected cells. If you want to detect senescence, we recommend using Senescence Detection Kit (ab65351).
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 250 tests Fixative Solution III 1 x 125ml Staining Solution II 1 x 125ml 100X Staining Supplement 1 x 1.5ml X-Gal 1 x 150mg -
研究分野
-
関連性
Beta galactosidase is a hydrolase enzyme that cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans. Beta galactosidase is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. Senescent cells display senescence-associated expression of beta galactosidase activity. -
細胞内局在
Isoform 1: Lysosome. Isoform 2: Cytoplasm, perinuclear region. Note=Localized to the perinuclear area of the cytoplasm but not to lysosomes. -
別名
- Acid beta galactosidase
- Beta galactosidase 1
- EBP
see all
画像
-
Gamma-radiation induced senescence in hFOB cells. The number of senescence-associated-β-galactosidase positive cells (left panel) cleary increases 72h after 8 Gy γ irradiation. Representative data was from one of a total of 3 indepent experiments. Image obtained from Li XH et al; PLOS One; 2012;7(5):e36604
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (15)
ab102534 は 15 報の論文で使用されています。
- Miller SP et al. A Proteomic Approach Identifies Isoform-Specific and Nucleotide-Dependent RAS Interactions. Mol Cell Proteomics 21:100268 (2022). PubMed: 35839996
- Pan W et al. Deubiquitinating enzyme USP30 negatively regulates mitophagy and accelerates myocardial cell senescence through antagonism of Parkin. Cell Death Discov 7:187 (2021). PubMed: 34290230
- Costantini M et al. Biofabricating murine and human myo-substitutes for rapid volumetric muscle loss restoration. EMBO Mol Med 13:e12778 (2021). PubMed: 33587336
- Ramakrishnan RK et al. Enhanced mitophagy in bronchial fibroblasts from severe asthmatic patients. PLoS One 15:e0242695 (2020). PubMed: 33253229
- Eakin AJ et al. Circulating Levels of Epirubicin Cause Endothelial Senescence While Compromising Metabolic Activity and Vascular Function. Front Cell Dev Biol 8:799 (2020). PubMed: 32974345